Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
maltose | above 20 mM, substrate inhibition | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.807 | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Hordeum vulgare | 2 alpha-D-glucose | - |
? | |
maltose + H2O | - |
Hordeum vulgare | alpha-D-glucose + D-glucose | - |
? | |
nigerose + H2O | - |
Hordeum vulgare | 2 alpha-D-glucose | - |
? | |
trehalose + H2O | low activity | Hordeum vulgare | alpha-D-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
begins to denature at, at pH 4 | Hordeum vulgare |
45 | - |
begins to denature at, at pH 5-6 | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | hydrolysis of maltose, recombinant enzyme | Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
most thermolabile at, high concentrations of sucrose protect from inactivation | Hordeum vulgare |