Activating Compound | Comment | Organism | Structure |
---|---|---|---|
n-Propanol | maximal stimulation at 7.5% | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
proteolytic treatment of chemically denatured enzyme generated by guanidine hydrochloride pretreatment results in complete maltase degradation | Saccharolobus solfataricus |
treatment with trypsin or chymotrypsin at 30°C or proteinase K at 30°C or at 55°C for 20 min in 40 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, pH 7.6, 8 mM magnesium acetate, 0.3 mM EDTA, 2 mM dithiothreitol, 10 mM sodium chloride and 20 mM potassium chloride has no effect | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
maltose | - |
Saccharolobus solfataricus | |
3.2 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
80000 | - |
5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
400000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% | Saccharolobus solfataricus |
n-propanol | 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120900 | - |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextrin + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
isomaltose + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-glucose | - |
? | |
maltoheptaose + H2O | - |
Saccharolobus solfataricus | maltohexaose + D-glucose | - |
? | |
maltose + H2O | - |
Saccharolobus solfataricus | alpha-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Saccharolobus solfataricus | alpha-D-glucose | - |
? | |
maltotriose + H2O | - |
Saccharolobus solfataricus | maltose + D-glucose | - |
? | |
methyl-alpha-glucoside + H2O | - |
Saccharolobus solfataricus | methanol + alpha-D-glucose | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucose | - |
? | |
starch + H2O | no activity | Saccharolobus solfataricus | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
tetramer | 4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 115 | 65°C: about 30% of maximal activity, 115°C: about 25% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life: 11 h | Saccharolobus solfataricus |
95 | - |
half-life: 3 h | Saccharolobus solfataricus |
100 | - |
half-life: 2.75 h | Saccharolobus solfataricus |
105 | - |
half-life: 11 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | about 50% of maximal activity at pH 3.5 and at pH 6.5 | Saccharolobus solfataricus |