Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | 0.4% w/v, bovine serum albumin, substantially increases activity | Thermococcus sp. | |
dithiothreitol | 1%, substantially increases activity | Thermococcus sp. |
General Stability | Organism |
---|---|
relatively unaffected by denaturing agents at 75°C | Thermococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight decrease of activity | Thermococcus sp. | |
Co2+ | - |
Thermococcus sp. | |
Hg2+ | - |
Thermococcus sp. | |
Mg2+ | slight decrease of activity | Thermococcus sp. | |
Ni2+ | - |
Thermococcus sp. | |
Zn2+ | slight decrease of activity | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
p-nitrophenyl-alpha-D-glucoside | free enzyme | Thermococcus sp. | |
0.56 | - |
p-nitrophenyl-alpha-D-glucoside | enzyme immobilized on oxirane acrylic beads | Thermococcus sp. | |
0.71 | - |
p-nitrophenyl-alpha-D-glucoside | cocross-linked enzyme | Thermococcus sp. | |
3 | - |
p-nitrophenyl-alpha-D-glucoside | enzyme immobilized on epoxy carrier | Thermococcus sp. | |
8.2 | - |
p-nitrophenyl-alpha-D-glucoside | enzyme immobilized on uncoated controlled glass beads | Thermococcus sp. | |
8.6 | - |
p-nitrophenyl-alpha-D-glucoside | enzyme immobilized on alkylamino glass beads | Thermococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermococcus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Thermococcus sp. |
120000 | - |
x * 120000, SDS-PAGE | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
- |
- |
Thermococcus sp. AN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Thermococcus sp. | 2 alpha-D-glucose | - |
? | |
isomaltose + H2O | - |
Thermococcus sp. AN1 | 2 alpha-D-glucose | - |
? | |
maltose + H2O | - |
Thermococcus sp. | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Thermococcus sp. AN1 | alpha-D-glucose + D-glucose | - |
? | |
nigerose + H2O | - |
Thermococcus sp. | 2 alpha-D-glucose | - |
? | |
nigerose + H2O | - |
Thermococcus sp. AN1 | 2 alpha-D-glucose | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Thermococcus sp. | 4-nitrophenol + D-glucose | - |
? | |
palatinose + H2O | - |
Thermococcus sp. | alpha-D-glucose + D-fructose | - |
? | |
palatinose + H2O | - |
Thermococcus sp. AN1 | alpha-D-glucose + D-fructose | - |
? | |
panose + H2O | - |
Thermococcus sp. | maltose + alpha-D-glucose | - |
? | |
panose + H2O | - |
Thermococcus sp. AN1 | maltose + alpha-D-glucose | - |
? | |
turanose + H2O | - |
Thermococcus sp. | alpha-D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
half-life: 35 min, in presence of 1% w/v dithiothreitol and 1% bovine serum albumin: 215 min | Thermococcus sp. |
110 | - |
half-life: 4 min | Thermococcus sp. |
130 | - |
in presence of 90% w/v sorbitol, 1% w/v dithiothreitol and 1% w/v bovine serum albumin, half-life: 30-60 min | Thermococcus sp. |