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Literature summary for 3.2.1.20 extracted from
- Purification and partial characterization of three forms of alpha-glucosidase from the fruit fly Drosophila melanogaster (1979), J. Biochem., 85, 123-130.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
p-nitrophenyl-alpha-D-glucoside | alpha-glucosidase III | Drosophila melanogaster |  |
| 0.77 | - |
p-nitrophenyl-alpha-D-glucoside | alpha-glucosidase II | Drosophila melanogaster | |
| 8.9 | - |
sucrose | alpha-glucosidase II | Drosophila melanogaster | |
| 10 | - |
sucrose | alpha-glucosidase III | Drosophila melanogaster | |
| 13.1 | - |
sucrose | alpha-glucosidase I | Drosophila melanogaster | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
alpha-glucosidase II, gel filtration | Drosophila melanogaster |
| 75000 | - |
1 * 75000, alpha-glucosidase III, SDS-PAGE | Drosophila melanogaster |
| 76000 | - |
alpha-glucosidase III, gel filtration | Drosophila melanogaster |
| 93000 | - |
2 * 93000, alpha-glucosidase I, SDS-PAGE | Drosophila melanogaster |
| 200000 | - |
alpha-glucosidase I, gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | alpha-glucosidase II is a glycoprotein | Drosophila melanogaster |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| alpha-glucosidase I, II, and II | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Drosophila melanogaster | 4-nitrophenol + D-glucose | - |
? | |
| sucrose + H2O | - |
Drosophila melanogaster | D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 93000, alpha-glucosidase I, SDS-PAGE | Drosophila melanogaster |
| monomer | 1 * 75000, alpha-glucosidase III, SDS-PAGE | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
alpha-glucosidase II with sucrose as substrate | Drosophila melanogaster |
| 5.5 | - |
p-nitrophenyl-alpha-D-glucoside, alpha-glucosidase II | Drosophila melanogaster |
| 6 | - |
alpha-glucosidase I and III with sucrose as substrate | Drosophila melanogaster |
| 6.5 | - |
p-nitrophenyl-alpha-D-glucoside, alpha-glucosidase III | Drosophila melanogaster |
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