Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.20 extracted from

  • Schönert, S.; Buder, T.; Dahl, M.K.
    Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis (1998), J. Bacteriol., 180, 2574-2578.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.135
-
 maltose
-
 Bacillus subtilis
0.21
-
 p-nitrophenyl-alpha-D-glucoside
-
 Bacillus subtilis
0.455
-
 isomaltose
-
 Bacillus subtilis
10.2
-
 sucrose
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis maltose is required for induction. The enzyme may play a role in the breakdown of internal storage polysaccharides containing alpha-1,4 and alpha-1,6 bonds ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
4°C, stable for at least 4 weeks Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
 Bacillus subtilis 2 alpha-D-glucose
-
 ?
maltose + H2O
-
 Bacillus subtilis alpha-D-glucose + D-glucose
-
 ?
additional information the enzyme is a specific oligo-1,4-1,6-alpha-glucosidase Bacillus subtilis ?
-
 ?
additional information maltose is required for induction. The enzyme may play a role in the breakdown of internal storage polysaccharides containing alpha-1,4 and alpha-1,6 bonds Bacillus subtilis ?
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Bacillus subtilis 4-nitrophenol + D-glucose
-
 ?
sucrose + H2O
-
 Bacillus subtilis D-fructose + alpha-D-glucose
-
 ?