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Literature summary for 3.2.1.2 extracted from

  • Comfort, D.A.; Chou, C.J.; Conners, S.B.; VanFossen, A.L.; Kelly, R.M.
    Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus (2008), Appl. Environ. Microbiol., 74, 1281-1283.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0827
-
 4-nitrophenyl alpha-maltopyranoside pH 7.0, 90°C Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the presence or absence of various divalent cations or EDTA had a minimal effect on enzyme activity Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U2G5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl alpha-maltopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-maltopyranose
-
 ?
glycogen + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?
maltose + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?
additional information the enzyme does not hydrolyze starch, glycogen, pullulan or large maltooligosaccharides Pyrococcus furiosus ?
-
 ?
starch + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-amylase
-
 Pyrococcus furiosus
PF0870
-
 Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
962
-
 4-nitrophenyl alpha-maltopyranoside pH 7.0, 90°C Pyrococcus furiosus