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Literature summary for 3.2.1.2 extracted from
- Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic beta-amylase from Calystegia sepium (hedge bindweed) rhizomes (2001), Eur. J. Biochem., 268, 6263-6273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Calystegia sepium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyclohexaamylose | iodine staining method, competitive inhibition | Calystegia sepium |  |
| D-glucose | p-nitrophenylmaltopentaoside hydrolysis: 125 mM, 87.5% inhibition, iodine staining method: mixed-type, weak inhibition compared with maltose and cyclohexaamylose | Calystegia sepium | |
| D-maltose | - |
Calystegia sepium | |
| D-mannose | 125 mM, 6% inhibition, p-nitrophenylmaltopentaoside hydrolysis | Calystegia sepium | |
| maltose | p-nitrophenylmaltopentaoside hydrolysis: 125 mM, 87.5% inhibition, iodine staining method: competitive inhibition | Calystegia sepium | |
| additional information | not inhibited by 250 mM lactose | Calystegia sepium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | exclusively located in | Calystegia sepium | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, SDS-PAGE, 4 * 56068, mass spectrometry, 4 * 56204, sequence calculation inclusive the N-terminal methionine | Calystegia sepium |
| 56068 | - |
4 * 55000, SDS-PAGE, 4 * 56068, mass spectrometry, 4 * 56204, sequence calculation inclusive the N-terminal methionine | Calystegia sepium |
| 56204 | - |
4 * 55000, SDS-PAGE, 4 * 56068, mass spectrometry, 4 * 56204, sequence calculation inclusive the N-terminal methionine | Calystegia sepium |
| 200000 | - |
gel filtration | Calystegia sepium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Calystegia sepium | rhizome beta-amylase is a cytoplasmic vegetative storage protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Calystegia sepium | Q9FQ07 | hedge bindweed, collected in Leuven in winter | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | apart from the removal of the N-terminal methionine no co- or posttranslational processing | Calystegia sepium |
| no glycoprotein | - |
Calystegia sepium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Calystegia sepium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| rhizome | resting, localized in the cortex and the pith of rhizomes, but not in vascular tissues, pericycle, endodermis and rhizodermis | Calystegia sepium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | catalyzes the release of maltose residues, amylopectin and starch are better substrates than amylose | Calystegia sepium | maltose + ? | - |
? | |
| amylose + H2O | catalyzes the release of maltose residues, less good substrate than starch and amylopectin | Calystegia sepium | maltose + ? | - |
? | |
| additional information | rhizome beta-amylase is a cytoplasmic vegetative storage protein | Calystegia sepium | ? | - |
? | |
| additional information | beta-amylase exclusively catalyzes the release of beta-maltose from the non-reducing ends of alpha-1,4-linked oligo- and polyglucans, three-dimensional structure | Calystegia sepium | ? | - |
? | |
| p-nitrophenylmaltopentaoside + H2O | catalyzes the release of p-nitrophenol, specific substrate | Calystegia sepium | p-nitrophenol + maltopentaose | - |
? | |
| starch + H2O | catalyzes the release of maltose residues, starch and amylopectin are better substrates than amylose | Calystegia sepium | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 55000, SDS-PAGE, 4 * 56068, mass spectrometry, 4 * 56204, sequence calculation inclusive the N-terminal methionine | Calystegia sepium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at, starch, amylose or amylopectin as substrate | Calystegia sepium |
| 40 | - |
assay at, p-nitrophenylmaltopentaoside as substrate | Calystegia sepium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Calystegia sepium |
| 70 | - |
10 min, complete inactivation | Calystegia sepium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Calystegia sepium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | active in the range | Calystegia sepium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | stable in the range | Calystegia sepium |
| 3 | 12 | irreversible inactivation below pH 3 and above pH 12 | Calystegia sepium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
cyclohexaamylose | pH 5, 20°C, iodine staining method | Calystegia sepium | |
| 11.7 | - |
D-maltose | pH 5, 20°C, iodine staining method | Calystegia sepium | |
| 262 | - |
D-glucose | pH 5, 20°C, iodine staining method | Calystegia sepium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Calystegia sepium | isoelectric focusing | - |
4.9 |
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