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Literature summary for 3.2.1.2 extracted from
- Purification and characterization of a novel thermostable beta-amylase from Clostridium thermosulphurogenes (1988), Biochem. J., 254, 835-840.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-cyclodextrin | - |
Thermoanaerobacterium thermosulfurigenes |  |
| beta-cyclodextrin | - |
Thermoanaerobacterium thermosulfurigenes | |
| PCMB | - |
Thermoanaerobacterium thermosulfurigenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km: 2.29 mg/ml for soluble starch at 60°C, 1.68 mg/ml for soluble starch at 75°C | Thermoanaerobacterium thermosulfurigenes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoanaerobacterium thermosulfurigenes | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
4 * 51000, SDS-PAGE | Thermoanaerobacterium thermosulfurigenes |
| 210000 | - |
gel filtration | Thermoanaerobacterium thermosulfurigenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoanaerobacterium thermosulfurigenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4215 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose + ? | - |
? | |
| amylose + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose | - |
? | |
| glycogen + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose | - |
? | |
| additional information | no activity with pullulan | Thermoanaerobacterium thermosulfurigenes | ? | - |
? | |
| starch + H2O | soluble | Thermoanaerobacterium thermosulfurigenes | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 51000, SDS-PAGE | Thermoanaerobacterium thermosulfurigenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 55% of maximal activity, 80°C: about 65% of maximal activity | Thermoanaerobacterium thermosulfurigenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stability is greatly enhanced by addition of 5 mM CaCl2 | Thermoanaerobacterium thermosulfurigenes |
| 70 | - |
2 h, stable in absence of substrate or Ca2+ | Thermoanaerobacterium thermosulfurigenes |
| 80 | - |
unstable in absence of substrate or Ca2+, stable in presence of 5 mM Ca2+ or 1% substrate | Thermoanaerobacterium thermosulfurigenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | pH 2.0: about 35% of maximal activity, pH 8.0: about 30% of maximal activity | Thermoanaerobacterium thermosulfurigenes |
| 5 | 6 | more than 80% of maximal activity at pH 5.0 and at pH 6.0 | Thermoanaerobacterium thermosulfurigenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | 70°C, in presence of 5 mM CaCl2 | Thermoanaerobacterium thermosulfurigenes |
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