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Literature summary for 3.2.1.2 extracted from
- beta-Amylases (1960), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 4, 345-368.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Hordeum vulgare |  |
| Ag+ | 0.001-0.1 mM AgNO3 | Ipomoea batatas | |
| Cu2+ | - |
Hordeum vulgare | |
| Cu2+ | 1 mM CuSO4, 50% inhibition | Ipomoea batatas | |
| Cu2+ | - |
Secale cereale | |
| Hg2+ | - |
Hordeum vulgare | |
| Hg2+ | 0.001-0.1 mM HgCl2 | Ipomoea batatas | |
| iodoacetic acid | partial | Glycine max | |
| Iodosobenzoate | - |
Ipomoea batatas | |
| NaCN | 0.1% | Glycine max | |
| NaNO2 | partial | Glycine max | |
| PCMB | - |
Glycine max | |
| PCMB | - |
Hordeum vulgare | |
| PCMB | 0.001-0.1 mM, inactivation is partly reversed by adding 10fold to 20fold excess of glutathione or 1,2-dithiopropanol | Ipomoea batatas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 152000 | - |
calculation from sedimentation and diffusion data | Ipomoea batatas |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Triticum aestivum | - |
? | - |
? | |
| additional information | Hordeum vulgare | germination markedly increases the beta-amylase | ? | - |
? | |
| additional information | Secale cereale | germination markedly increases the beta-amylase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
| Hordeum vulgare | - |
- |
- |
| Ipomoea batatas | - |
- |
- |
| Secale cereale | - |
- |
- |
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Triticum aestivum | ? | - |
? | |
| additional information | germination markedly increases the beta-amylase | Hordeum vulgare | ? | - |
? | |
| additional information | germination markedly increases the beta-amylase | Secale cereale | ? | - |
? | |
| starch + H2O | - |
Triticum aestivum | maltose + ? | - |
? | |
| starch + H2O | - |
Secale cereale | maltose + ? | - |
? | |
| starch + H2O | soluble | Hordeum vulgare | maltose + ? | - |
? | |
| starch + H2O | soluble | Glycine max | maltose + ? | - |
? | |
| starch + H2O | soluble | Ipomoea batatas | maltose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Ipomoea batatas |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Ipomoea batatas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | acetate buffer | Ipomoea batatas |
| 5.3 | - |
- |
Triticum aestivum |
| 5.5 | 6 | citrate buffer | Ipomoea batatas |
| 6 | - |
- |
Glycine max |
| 6 | - |
- |
Ipomoea batatas |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | pH 3.0: about 35% of maximal activity, pH 8.0: about 70% of maximal activity | Glycine max |
| 3 | 8 | pH 3.0: about 70% of maximal activity, pH 8.0: about 60% of maximal activity | Triticum aestivum |
| 3 | 8.5 | pH 3.0: about 30% of maximal activity, pH 8.5: about 40% of maximal activity | Triticum aestivum |
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