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Literature summary for 3.2.1.198 extracted from

  • Hakki, Z.; Thompson, A.J.; Bellmaine, S.; Speciale, G.; Davies, G.J.; Williams, S.J.
    Structural and kinetic dissection of the endo-alpha-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp. (2015), Chemistry, 21, 1966-1977.
    View publication on PubMed
Search for more literature for 3.2.1.198

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitor alpha-mannopyranosyl-1,3-isofagomine. The inhibitor is coordinated by residues Y46, W48, H63, W126, E156, E333 and E336 Bacteroides xylanisolvens

Protein Variants

Protein Variants Comment Organism
E333Q inactive Bacteroides xylanisolvens

Inhibitors

Inhibitors Comment Organism Structure
alpha-mannopyranosyl-1,3-isofagomine Kd value 140 nm Bacteroides thetaiotaomicron
alpha-mannopyranosyl-1,3-isofagomine Kd value 140 nm Bacteroides xylanisolvens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
0.404
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
1.83
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
2.6
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A109 GH99 family protein
-
Bacteroides thetaiotaomicron DSM 2079 Q8A109 GH99 family protein
-
Bacteroides xylanisolvens D6D1V7 GH99 family protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides xylanisolvens ?
-
 ?
additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides thetaiotaomicron ?
-
 ?
additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?

Synonyms

Synonyms Comment Organism
BXY_34140
-
 Bacteroides xylanisolvens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
0.0008
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
0.0015
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
3
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron

General Information

General Information Comment Organism
physiological function enzyme releases alpha-1,3-mannobiose by hydrolyzing alphaMan-1,3-aMan->1,2-alphaMan-1,2-alphaMan sequences within branches off the main alpha-1,6-mannan backbone of fungal cell walls Bacteroides xylanisolvens
physiological function enzyme releases alpha-1,3-mannobiose by hydrolyzing alphaMan-1,3-aMan->1,2-alphaMan-1,2-alphaMan sequences within branches off the main alpha-1,6-mannan backbone of fungal cell walls Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00062
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
0.0021
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
0.0085
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
1.15
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron