Literature summary for 3.2.1.194 extracted from

Shin, K.; Oh, D.
Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides (2016), Crit. Rev. Biotechnol., 36, 1036-1049 .

Search for more literature for 3.2.1.194

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ginsenoside R1 + H2O	Aspergillus sp.	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Re + H2O	Pyrococcus furiosus	-	ginsenoside Rg1 + L-rhamnose	-	?
ginsenoside Re + H2O	Aspergillus sp.	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Re + H2O	Penicillium decumbens	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Rf + H2O	Aspergillus niger	-	ginsenoside Rh1 + D-glucose	-	?
ginsenoside Rf + H2O	Pyrococcus furiosus	-	ginsenoside Rh1 + D-glucose	-	?
ginsenoside Rf + H2O	Aspergillus sp.	-	protopanaxatriol + 2 D-glucose	-	?
ginsenoside Rf + H2O	Penicillium decumbens	-	protopanaxatriol + 2 D-glucose	-	?
ginsenoside Rg1 + H2O	Penicillium decumbens	-	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	Fusarium subglutinans	-	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	Penicillium sclerotiorum	-	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	Sanguibacter keddieii	-	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg2 + 2 H2O	Penicillium decumbens	-	protopanaxatriol + D-glucose + L-rhamnose	-	?
ginsenoside Rg2 + H2O	Pyrococcus furiosus	-	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	Thermoanaerobacterium thermosaccharolyticum	-	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	Absidia sp. (in: Fungi)	-	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	Aspergillus sp.	-	protopanaxatriol + D-glucose + L-rhamnose	-	?
ginsenoside Rh1 + H2O	Penicillium decumbens	-	protopanaxatriol + D-glucose	-	?
additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	?	-	?
additional information	Aspergillus niger	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Aspergillus niger	?	-	?
additional information	Absidia sp. (in: Fungi)	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	?	-	?
additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion was performed using b-glycosidase from Pyrococcus furiosus	?	-	?
additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Thermoanaerobacterium thermosaccharolyticum	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Penicillium decumbens	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Fusarium subglutinans	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Penicillium sclerotiorum	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Sanguibacter keddieii	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
additional information	Aspergillus sp.	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	?	-	?
notoginsenoside R1 + H2O	Pyrococcus furiosus	-	ginsenoside Rg1 + D-xylose	-	?
notoginsenoside R1 + H2O	Thermoanaerobacterium thermosaccharolyticum	-	ginsenoside Rg1 + D-xylose	-	?

Organism

Organism	UniProt	Comment	Textmining
Absidia sp. (in: Fungi)	-	-	-
Aspergillus niger	-	-	-
Aspergillus sp.	-	-	-
Fusarium subglutinans	-	-	-
Penicillium decumbens	-	-	-
Penicillium sclerotiorum	-	-	-
Pyrococcus furiosus	-	-	-
Sanguibacter keddieii	-	-	-
Thermoanaerobacterium thermosaccharolyticum	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1	-	pH 5.0, 40°C	Absidia sp. (in: Fungi)
2	-	pH 5.5, 95°C	Pyrococcus furiosus
2	-	substrate ginsenoside Rf, pH 6.0, 40°C	Aspergillus sp.
4	-	substrate ginsenoside Rf, pH 7.5, 37°C	Aspergillus niger
4.5	-	pH 6.5, 60°C	Thermoanaerobacterium thermosaccharolyticum
136	-	pH 6.0, 50°C	Fusarium subglutinans
136	-	pH 6.0, 50°C	Penicillium sclerotiorum
912	-	pH 6.0, 40°C	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ginsenoside R1 + H2O	-	Aspergillus sp.	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Re + H2O	-	Pyrococcus furiosus	ginsenoside Rg1 + L-rhamnose	-	?
ginsenoside Re + H2O	-	Aspergillus sp.	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Re + H2O	-	Penicillium decumbens	ginsenoside F1 + D-glucose + L-rhamnose	-	?
ginsenoside Rf + H2O	-	Aspergillus niger	ginsenoside Rh1 + D-glucose	-	?
ginsenoside Rf + H2O	-	Pyrococcus furiosus	ginsenoside Rh1 + D-glucose	-	?
ginsenoside Rf + H2O	-	Aspergillus sp.	protopanaxatriol + 2 D-glucose	-	?
ginsenoside Rf + H2O	-	Penicillium decumbens	protopanaxatriol + 2 D-glucose	-	?
ginsenoside Rg1 + H2O	-	Penicillium decumbens	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	-	Fusarium subglutinans	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	-	Penicillium sclerotiorum	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg1 + H2O	-	Sanguibacter keddieii	ginsenoside F1 + D-glucose	-	?
ginsenoside Rg2 + 2 H2O	-	Penicillium decumbens	protopanaxatriol + D-glucose + L-rhamnose	-	?
ginsenoside Rg2 + H2O	-	Pyrococcus furiosus	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	-	Thermoanaerobacterium thermosaccharolyticum	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	-	Absidia sp. (in: Fungi)	ginsenoside Rh1 + L-rhamnose	-	?
ginsenoside Rg2 + H2O	-	Aspergillus sp.	protopanaxatriol + D-glucose + L-rhamnose	-	?
ginsenoside Rh1 + H2O	-	Penicillium decumbens	protopanaxatriol + D-glucose	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Aspergillus niger	Aspergillus niger	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Absidia sp. (in: Fungi)	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion was performed using b-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Thermoanaerobacterium thermosaccharolyticum	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium decumbens	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Fusarium subglutinans	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium sclerotiorum	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Sanguibacter keddieii	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	Aspergillus sp.	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion is performed using beta-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Gglycosidase from Pyrococcus furiosus shows broad substrate specificity that hydrolyzes the outer residue glucose and rhamnose and xylose at C-6	Pyrococcus furiosus	?	-	?
additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Xylosidase from Thermoanaerobacterium thermosaccharolyticum converts notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1 by selectively hydrolyzing only the outer xylose at C-6	Thermoanaerobacterium thermosaccharolyticum	?	-	?
notoginsenoside R1 + H2O	-	Pyrococcus furiosus	ginsenoside Rg1 + D-xylose	-	?
notoginsenoside R1 + H2O	-	Thermoanaerobacterium thermosaccharolyticum	ginsenoside Rg1 + D-xylose	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000	Penicillium sclerotiorum
?	x * 56000	Aspergillus sp.
?	x * 53000	Absidia sp. (in: Fungi)
?	x * 59000	Thermoanaerobacterium thermosaccharolyticum
?	x * 54000	Pyrococcus furiosus
?	x * 67000	Sanguibacter keddieii
?	x * 85000	Fusarium subglutinans
?	x * 93000	Aspergillus niger

Synonyms

Synonyms	Comment	Organism
alpha-L-rhamnosidase	-	Absidia sp. (in: Fungi)
beta-glucosidase	-	Pyrococcus furiosus
beta-glucosidase	-	Fusarium subglutinans
beta-glucosidase	-	Penicillium sclerotiorum
beta-glucosidase	-	Sanguibacter keddieii
beta-glycosidase	-	Aspergillus niger
beta-xylosidase	-	Thermoanaerobacterium thermosaccharolyticum
ginsenosidase type IV	-	Aspergillus sp.
ginsenosidase type IV-i-1	-	Aspergillus niger
ginsenosidase type IV-i-1	-	Pyrococcus furiosus
ginsenosidase type IV-i-2	-	Pyrococcus furiosus
ginsenosidase type IV-i-2	-	Absidia sp. (in: Fungi)
ginsenosidase type IV-i-3	-	Pyrococcus furiosus
ginsenosidase type IV-i-3	-	Thermoanaerobacterium thermosaccharolyticum
ginsenosidase type IV-ii	-	Aspergillus sp.
ginsenosidase type IV-ii	-	Penicillium decumbens
ginsenosidase type IV-ii	-	Fusarium subglutinans
ginsenosidase type IV-ii	-	Penicillium sclerotiorum
ginsenosidase type IV-ii	-	Sanguibacter keddieii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Sanguibacter keddieii
37	-	-	Aspergillus niger
40	-	-	Aspergillus sp.
40	-	-	Absidia sp. (in: Fungi)
50	-	-	Fusarium subglutinans
60	-	-	Thermoanaerobacterium thermosaccharolyticum
65	-	-	Penicillium sclerotiorum
95	-	-	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	-	Penicillium sclerotiorum
5	-	-	Absidia sp. (in: Fungi)
5.5	-	-	Pyrococcus furiosus
6	-	-	Aspergillus sp.
6	-	-	Fusarium subglutinans
6.5	-	-	Thermoanaerobacterium thermosaccharolyticum
7.5	-	-	Aspergillus niger
8	-	-	Sanguibacter keddieii

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Pyrococcus furiosus
evolution	the enzyme belongs to the glycosyl hydrolase family 39, GH39	Thermoanaerobacterium thermosaccharolyticum
evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Aspergillus niger
evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Aspergillus sp.
evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Sanguibacter keddieii
evolution	the enzyme belongs to the glycosyl hydrolase family 78, GH78	Absidia sp. (in: Fungi)
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Aspergillus niger
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Pyrococcus furiosus
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Pyrococcus furiosus
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Absidia sp. (in: Fungi)
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Pyrococcus furiosus
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Thermoanaerobacterium thermosaccharolyticum
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium decumbens
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Fusarium subglutinans
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium sclerotiorum
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Sanguibacter keddieii
physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	Aspergillus sp.