Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain ER2566 | Clavibacter michiganensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside Rf + H2O | Clavibacter michiganensis | - |
ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rf + H2O | Clavibacter michiganensis DSM 46364 | - |
ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | Clavibacter michiganensis | - |
ginsenoside F1 + D-glucose | - |
? | |
ginsenoside Rh1 + H2O | Clavibacter michiganensis | - |
protopanaxatriol aglycone + D-glucose | - |
? | |
additional information | Clavibacter michiganensis | the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively | ? | - |
? | |
additional information | Clavibacter michiganensis DSM 46364 | the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clavibacter michiganensis | - |
- |
- |
Clavibacter michiganensis DSM 46364 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside Rf + H2O | - |
Clavibacter michiganensis | ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rf + H2O | - |
Clavibacter michiganensis DSM 46364 | ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | - |
Clavibacter michiganensis | ginsenoside F1 + D-glucose | - |
? | |
ginsenoside Rh1 + H2O | - |
Clavibacter michiganensis | protopanaxatriol aglycone + D-glucose | - |
? | |
additional information | the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively | Clavibacter michiganensis | ? | - |
? | |
additional information | recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position | Clavibacter michiganensis | ? | - |
? | |
additional information | the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively | Clavibacter michiganensis DSM 46364 | ? | - |
? | |
additional information | recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position | Clavibacter michiganensis DSM 46364 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.2.1.191 | Clavibacter michiganensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Clavibacter michiganensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Clavibacter michiganensis |
General Information | Comment | Organism |
---|---|---|
additional information | the amino acid at position 512 in beta-glucosidase from Clavibacter michiganensis determines the regioselectivity for hydrolyzing gypenoside XVII, obtained from the GypXVII-docked homology models of beta-glucosidase. Homology modeling of Clavibacter michiganensis beta-glucosidase based on the crystal structure of beta-D-glucan exohydrolase from Hordeum vulgare (PDB ID 1EX1) as a template | Clavibacter michiganensis |