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Literature summary for 3.2.1.194 extracted from

  • Shin, K.C.; Hong, S.H.; Seo, M.J.; Oh, D.K.
    An amino acid at position 512 in beta-glucosidase from Clavibacter michiganensis determines the regioselectivity for hydrolyzing gypenoside XVII (2015), Appl. Microbiol. Biotechnol., 99, 7987-7996 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain ER2566 Clavibacter michiganensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rf + H2O Clavibacter michiganensis
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ginsenoside Rh1 + D-glucose
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?
ginsenoside Rf + H2O Clavibacter michiganensis DSM 46364
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ginsenoside Rh1 + D-glucose
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?
ginsenoside Rg1 + H2O Clavibacter michiganensis
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ginsenoside F1 + D-glucose
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?
ginsenoside Rh1 + H2O Clavibacter michiganensis
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protopanaxatriol aglycone + D-glucose
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?
additional information Clavibacter michiganensis the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively ?
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?
additional information Clavibacter michiganensis DSM 46364 the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively ?
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?

Organism

Organism UniProt Comment Textmining
Clavibacter michiganensis
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Clavibacter michiganensis DSM 46364
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rf + H2O
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Clavibacter michiganensis ginsenoside Rh1 + D-glucose
-
?
ginsenoside Rf + H2O
-
Clavibacter michiganensis DSM 46364 ginsenoside Rh1 + D-glucose
-
?
ginsenoside Rg1 + H2O
-
Clavibacter michiganensis ginsenoside F1 + D-glucose
-
?
ginsenoside Rh1 + H2O
-
Clavibacter michiganensis protopanaxatriol aglycone + D-glucose
-
?
additional information the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively Clavibacter michiganensis ?
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?
additional information recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position Clavibacter michiganensis ?
-
?
additional information the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively Clavibacter michiganensis DSM 46364 ?
-
?
additional information recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position Clavibacter michiganensis DSM 46364 ?
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?

Synonyms

Synonyms Comment Organism
More cf. EC 3.2.1.191 Clavibacter michiganensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Clavibacter michiganensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Clavibacter michiganensis

General Information

General Information Comment Organism
additional information the amino acid at position 512 in beta-glucosidase from Clavibacter michiganensis determines the regioselectivity for hydrolyzing gypenoside XVII, obtained from the GypXVII-docked homology models of beta-glucosidase. Homology modeling of Clavibacter michiganensis beta-glucosidase based on the crystal structure of beta-D-glucan exohydrolase from Hordeum vulgare (PDB ID 1EX1) as a template Clavibacter michiganensis