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Literature summary for 3.2.1.194 extracted from

  • Wang, D.M.; Yu, H.S.; Song, J.G.; Xu, Y.F.; Jin, F.X.
    Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides (2012), Proc. Biochem., 47, 133-138.
No PubMed abstract available
Search for more literature for 3.2.1.194

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.74
-
 ginsenoside Rg2 pH 6, 40°C Aspergillus sp.
7.06
-
 ginsenoside R1 pH 6, 40°C Aspergillus sp.
7.63
-
 ginsenoside Rg1 pH 6, 40°C Aspergillus sp.
9.43
-
 ginsenoside Rf pH 6, 40°C Aspergillus sp.
22.2
-
 ginsenoside Re pH 6, 40°C Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Aspergillus sp. 39g
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus sp.

Reaction

Reaction Comment Organism Reaction ID
a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide overall reaction Aspergillus sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 pH 6, 40°C Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside Aspergillus sp. ginsenoside Rg1 + D-xylose
-
 ?
ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside Aspergillus sp. 39g ginsenoside Rg1 + D-xylose
-
 ?
ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rg1 + L-rhamnose
-
 ?
ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. 39g ginsenoside Rg1 + L-rhamnose
-
 ?
ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. ginsenoside Rh1 + D-glucose
-
 ?
ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. 39g ginsenoside Rh1 + D-glucose
-
 ?
ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. ginsenoside F1 + D-glucose
-
 ?
ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. 39g ginsenoside F1 + D-glucose
-
 ?
ginsenoside Rg2 + H2O the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rh1 + L-rhamnose
-
 ?
ginsenoside Rh1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. protopanaxatriol + D-glucose
-
 ?
additional information the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside Aspergillus sp. ?
-
 ?
additional information the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside Aspergillus sp. 39g ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Aspergillus sp.