Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
compound Mc + H2O | Armillaria mellea | - |
compound K + L-arabinofuranose | - |
? | |
compound Mc + H2O | Armillaria mellea KACC 50013 | - |
compound K + L-arabinofuranose | - |
? | |
compound Mc1 + H2O | Armillaria mellea | - |
compound Mc + D-glucose | - |
? | |
ginsenoside F2 + H2O | Armillaria mellea | - |
compound K + D-glucose | - |
? | |
ginsenoside Rc + H2O | Pleurotus ostreatus | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Ganoderma lucidum | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Armillaria mellea | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Ganoderma applanatum | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Sanghuangporus baumii | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Armillaria mellea | - |
compound Mc1 + D-glucose | - |
? | |
ginsenoside Rc + H2O | Ganoderma lucidum KACC 42231 | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Armillaria mellea KACC 50013 | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Pleurotus ostreatus KACC 50356 | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Ganoderma applanatum KACC 53688 | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | Sanghuangporus baumii KACC 53719 | - |
ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rd + H2O | Armillaria mellea | - |
ginsenoside F2 + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armillaria mellea | - |
- |
- |
Armillaria mellea KACC 50013 | - |
- |
- |
Ganoderma applanatum | - |
- |
- |
Ganoderma applanatum KACC 53688 | - |
- |
- |
Ganoderma lucidum | - |
- |
- |
Ganoderma lucidum KACC 42231 | - |
- |
- |
Pleurotus ostreatus | - |
- |
- |
Pleurotus ostreatus KACC 50356 | - |
- |
- |
Sanghuangporus baumii | - |
- |
- |
Sanghuangporus baumii KACC 53719 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 33.8fold from mycelium by ammonium sulfate fractionation, anion exchange chromatographydialysis, lyophilization, and gel filtration | Armillaria mellea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Pleurotus ostreatus | - |
mycelium | - |
Ganoderma lucidum | - |
mycelium | - |
Armillaria mellea | - |
mycelium | - |
Ganoderma applanatum | - |
mycelium | - |
Sanghuangporus baumii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.9 | - |
substrate ginsenoside Rc, pH 4.5, 50°C | Armillaria mellea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-arabinofuranoside + H2O | 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Armillaria mellea | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl-alpha-L-arabinofuranoside + H2O | 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Armillaria mellea KACC 50013 | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Armillaria mellea | 4-nitrophenol + L-arabinopyranose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Armillaria mellea KACC 50013 | 4-nitrophenol + L-arabinopyranose | - |
? | |
4-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Armillaria mellea | 4-nitrophenol + D-glucopyranose | - |
? | |
compound Mc + H2O | - |
Armillaria mellea | compound K + L-arabinofuranose | - |
? | |
compound Mc + H2O | - |
Armillaria mellea KACC 50013 | compound K + L-arabinofuranose | - |
? | |
compound Mc1 + H2O | - |
Armillaria mellea | compound Mc + D-glucose | - |
? | |
ginsenoside F2 + H2O | - |
Armillaria mellea | compound K + D-glucose | - |
? | |
ginsenoside Rc + H2O | - |
Pleurotus ostreatus | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Ganoderma lucidum | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Armillaria mellea | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Ganoderma applanatum | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Sanghuangporus baumii | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Ganoderma lucidum KACC 42231 | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Armillaria mellea KACC 50013 | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Pleurotus ostreatus KACC 50356 | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Ganoderma applanatum KACC 53688 | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Sanghuangporus baumii KACC 53719 | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | - |
Armillaria mellea | compound Mc1 + D-glucose | - |
? | |
ginsenoside Rd + H2O | - |
Armillaria mellea | ginsenoside F2 + D-glucose | - |
? | |
additional information | enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside | Armillaria mellea | ? | - |
? | |
additional information | the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Ganoderma applanatum | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Pleurotus ostreatus | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Ganoderma lucidum | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Sanghuangporus baumii | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Ganoderma lucidum KACC 42231 | ? | - |
? | |
additional information | enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside | Armillaria mellea KACC 50013 | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Pleurotus ostreatus KACC 50356 | ? | - |
? | |
additional information | the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Ganoderma applanatum KACC 53688 | ? | - |
? | |
additional information | the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages | Sanghuangporus baumii KACC 53719 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 121500, SDS-PAGE | Armillaria mellea |
Synonyms | Comment | Organism |
---|---|---|
BG-1 | - |
Armillaria mellea |
ginsenoside Rc-hydrolyzing beta-glucosidase | - |
Pleurotus ostreatus |
ginsenoside Rc-hydrolyzing beta-glucosidase | - |
Ganoderma lucidum |
ginsenoside Rc-hydrolyzing beta-glucosidase | - |
Armillaria mellea |
ginsenoside Rc-hydrolyzing beta-glucosidase | - |
Ganoderma applanatum |
ginsenoside Rc-hydrolyzing beta-glucosidase | - |
Sanghuangporus baumii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K | Armillaria mellea |
55 | 60 | the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K | Armillaria mellea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Armillaria mellea |
General Information | Comment | Organism |
---|---|---|
evolution | Armillaria mellea beta-glucosidase exists in three isomeric forms, BG-1, BG-2 and BG-3, that they exhibit different retention behaviors on DEAE cellulose column and hydrolytic activity toward 4-nitrophenyl beta-D-glucopyranoside. Moreover, when ginsenosides Rc, Rb1 andRd are used as substrates, isozyme BG-1 shows different hydrolytic activity from those of isozymes BG-2 and BG-3 | Armillaria mellea |