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Literature summary for 3.2.1.193 extracted from

  • Upadhyaya, J.; Yoon, M.S.; Kim, M.J.; Ryu, N.S.; Song, Y.E.; Kim, Y.H.; Kim, M.K.
    Purification and characterization of a novel ginsenoside Rc-hydrolyzing beta-glucosidase from Armillaria mellea mycelia (2016), AMB Express, 6, 112 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
compound Mc + H2O Armillaria mellea
-
compound K + L-arabinofuranose
-
?
compound Mc + H2O Armillaria mellea KACC 50013
-
compound K + L-arabinofuranose
-
?
compound Mc1 + H2O Armillaria mellea
-
compound Mc + D-glucose
-
?
ginsenoside F2 + H2O Armillaria mellea
-
compound K + D-glucose
-
?
ginsenoside Rc + H2O Pleurotus ostreatus
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Ganoderma lucidum
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Armillaria mellea
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Ganoderma applanatum
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Sanghuangporus baumii
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Armillaria mellea
-
compound Mc1 + D-glucose
-
?
ginsenoside Rc + H2O Ganoderma lucidum KACC 42231
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Armillaria mellea KACC 50013
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Pleurotus ostreatus KACC 50356
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Ganoderma applanatum KACC 53688
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O Sanghuangporus baumii KACC 53719
-
ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rd + H2O Armillaria mellea
-
ginsenoside F2 + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Armillaria mellea
-
-
-
Armillaria mellea KACC 50013
-
-
-
Ganoderma applanatum
-
-
-
Ganoderma applanatum KACC 53688
-
-
-
Ganoderma lucidum
-
-
-
Ganoderma lucidum KACC 42231
-
-
-
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus KACC 50356
-
-
-
Sanghuangporus baumii
-
-
-
Sanghuangporus baumii KACC 53719
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 33.8fold from mycelium by ammonium sulfate fractionation, anion exchange chromatographydialysis, lyophilization, and gel filtration Armillaria mellea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Pleurotus ostreatus
-
mycelium
-
Ganoderma lucidum
-
mycelium
-
Armillaria mellea
-
mycelium
-
Ganoderma applanatum
-
mycelium
-
Sanghuangporus baumii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.9
-
substrate ginsenoside Rc, pH 4.5, 50°C Armillaria mellea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-arabinofuranoside + H2O 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea 4-nitrophenol + L-arabinofuranose
-
?
4-nitrophenyl-alpha-L-arabinofuranoside + H2O 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea KACC 50013 4-nitrophenol + L-arabinofuranose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea 4-nitrophenol + L-arabinopyranose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea KACC 50013 4-nitrophenol + L-arabinopyranose
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
-
Armillaria mellea 4-nitrophenol + D-glucopyranose
-
?
compound Mc + H2O
-
Armillaria mellea compound K + L-arabinofuranose
-
?
compound Mc + H2O
-
Armillaria mellea KACC 50013 compound K + L-arabinofuranose
-
?
compound Mc1 + H2O
-
Armillaria mellea compound Mc + D-glucose
-
?
ginsenoside F2 + H2O
-
Armillaria mellea compound K + D-glucose
-
?
ginsenoside Rc + H2O
-
Pleurotus ostreatus ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Ganoderma lucidum ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Armillaria mellea ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Ganoderma applanatum ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Sanghuangporus baumii ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Ganoderma lucidum KACC 42231 ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Armillaria mellea KACC 50013 ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Pleurotus ostreatus KACC 50356 ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Ganoderma applanatum KACC 53688 ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Sanghuangporus baumii KACC 53719 ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Armillaria mellea compound Mc1 + D-glucose
-
?
ginsenoside Rd + H2O
-
Armillaria mellea ginsenoside F2 + D-glucose
-
?
additional information enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside Armillaria mellea ?
-
?
additional information the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma applanatum ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Pleurotus ostreatus ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma lucidum ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Sanghuangporus baumii ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma lucidum KACC 42231 ?
-
?
additional information enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside Armillaria mellea KACC 50013 ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Pleurotus ostreatus KACC 50356 ?
-
?
additional information the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma applanatum KACC 53688 ?
-
?
additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Sanghuangporus baumii KACC 53719 ?
-
?

Subunits

Subunits Comment Organism
? x * 121500, SDS-PAGE Armillaria mellea

Synonyms

Synonyms Comment Organism
BG-1
-
Armillaria mellea
ginsenoside Rc-hydrolyzing beta-glucosidase
-
Pleurotus ostreatus
ginsenoside Rc-hydrolyzing beta-glucosidase
-
Ganoderma lucidum
ginsenoside Rc-hydrolyzing beta-glucosidase
-
Armillaria mellea
ginsenoside Rc-hydrolyzing beta-glucosidase
-
Ganoderma applanatum
ginsenoside Rc-hydrolyzing beta-glucosidase
-
Sanghuangporus baumii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K Armillaria mellea
55 60 the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K Armillaria mellea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
Armillaria mellea

General Information

General Information Comment Organism
evolution Armillaria mellea beta-glucosidase exists in three isomeric forms, BG-1, BG-2 and BG-3, that they exhibit different retention behaviors on DEAE cellulose column and hydrolytic activity toward 4-nitrophenyl beta-D-glucopyranoside. Moreover, when ginsenosides Rc, Rb1 andRd are used as substrates, isozyme BG-1 shows different hydrolytic activity from those of isozymes BG-2 and BG-3 Armillaria mellea