Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Aspergillus sp. | |
Pb2+ | - |
Aspergillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50-100 mM, slight activation | Aspergillus sp. | |
Mg2+ | 50-100 mM, slight activation | Aspergillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Aspergillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. | - |
- |
- |
Aspergillus sp. g48p | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose | Hydrolysis of the 3-O-beta-D-(1->2)-glucosidic bond, the 3-O-beta-D-glucopyranosyl bond and the 20-O-beta-D-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20 and no glucosyl or one glucosyl residue at position 3. Starting with a ginsenoside that is glycosylated at both positions (e.g. ginsenoside Rb1, Rb2, Rb3, Rc or Rd), the most common products are ginsenoside F2 and ginsenoside C-K with low amounts of ginsenoside Rh2. | Aspergillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
40°C, pH not specified in the publication, substrate: ginsenoside Rg3 | Aspergillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside Rb1 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside | Aspergillus sp. | ginsenoside F2 + 2 D-glucose | - |
? | |
ginsenoside Rb1 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside | Aspergillus sp. g48p | ginsenoside F2 + 2 D-glucose | - |
? | |
ginsenoside Rb2 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside | Aspergillus sp. | ginsenoside F2 + D-glucose + L-arabinopyranose | - |
? | |
ginsenoside Rb2 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + L-arabinopyranose | - |
? | |
ginsenoside Rb3 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside | Aspergillus sp. | ginsenoside F2 + D-glucose + D-xylose | - |
? | |
ginsenoside Rb3 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + D-xylose | - |
? | |
ginsenoside Rc + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside | Aspergillus sp. | ginsenoside F2 + D-glucose + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + L-arabinofuranose | - |
? | |
ginsenoside Rd + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside | Aspergillus sp. | ginsenoside + D-glucose | - |
? | |
additional information | the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 | Aspergillus sp. | ? | - |
? | |
additional information | the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 | Aspergillus sp. g48p | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Aspergillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Aspergillus sp. |
50 | - |
- |
Aspergillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 60% of maximal activity, 70°C: about 60% of maximal activity | Aspergillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 48 h, stable | Aspergillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH 4.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity | Aspergillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | stable | Aspergillus sp. |