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Literature summary for 3.2.1.193 extracted from

  • Yu, H.; Zhang, C.; Lu, M.; Sun, F.; Fu, Y.; Jin, F.
    Purification and characterization of new special ginsenosidase hydrolyzing multi-glycosides of protopanaxadiol ginsenosides, ginsenosidase type I (2007), Chem. Pharm. Bull., 55, 231-235.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Aspergillus sp.
Pb2+
-
Aspergillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 50-100 mM, slight activation Aspergillus sp.
Mg2+ 50-100 mM, slight activation Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Aspergillus sp. g48p
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus sp.

Reaction

Reaction Comment Organism Reaction ID
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose Hydrolysis of the 3-O-beta-D-(1->2)-glucosidic bond, the 3-O-beta-D-glucopyranosyl bond and the 20-O-beta-D-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20 and no glucosyl or one glucosyl residue at position 3. Starting with a ginsenoside that is glycosylated at both positions (e.g. ginsenoside Rb1, Rb2, Rb3, Rc or Rd), the most common products are ginsenoside F2 and ginsenoside C-K with low amounts of ginsenoside Rh2. Aspergillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
40°C, pH not specified in the publication, substrate: ginsenoside Rg3 Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rb1 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside Aspergillus sp. ginsenoside F2 + 2 D-glucose
-
?
ginsenoside Rb1 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside Aspergillus sp. g48p ginsenoside F2 + 2 D-glucose
-
?
ginsenoside Rb2 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside Aspergillus sp. ginsenoside F2 + D-glucose + L-arabinopyranose
-
?
ginsenoside Rb2 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside Aspergillus sp. g48p ginsenoside F2 + D-glucose + L-arabinopyranose
-
?
ginsenoside Rb3 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside Aspergillus sp. ginsenoside F2 + D-glucose + D-xylose
-
?
ginsenoside Rb3 + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside Aspergillus sp. g48p ginsenoside F2 + D-glucose + D-xylose
-
?
ginsenoside Rc + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside Aspergillus sp. ginsenoside F2 + D-glucose + L-arabinofuranose
-
?
ginsenoside Rc + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside Aspergillus sp. g48p ginsenoside F2 + D-glucose + L-arabinofuranose
-
?
ginsenoside Rd + H2O ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside Aspergillus sp. ginsenoside + D-glucose
-
?
additional information the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. ?
-
?
additional information the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. g48p ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Aspergillus sp.
50
-
-
Aspergillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 60% of maximal activity, 70°C: about 60% of maximal activity Aspergillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 48 h, stable Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity Aspergillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 stable Aspergillus sp.