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Literature summary for 3.2.1.192 extracted from

  • Renchinkhand, G.; Cho, S.H.; Urgamal, M.; Park, Y.W.; Nam, J.H.; Bae, H.C.; Song, G.Y.; Nam, M.S.
    Characterization of Paenibacillus sp. MBT213 isolated from raw milk and its ability to convert ginsenoside Rb1 into ginsenoside Rd from Panax ginseng (2017), Korean J. Food Sci. Anim. Resour., 37, 735-742 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the beta-glucosidase activity of Paenibacillus sp. MBT213 strain may be utilized in development of variety of health foods, dairy foods and pharmaceutical products Paenibacillus sp. MBT213

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rb1 + H2O Paenibacillus sp. MBT213
-
ginsenoside Rd + D-glucopyranose
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. MBT213
-
isolated from raw milk
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information crude enzyme of strain MBT213 exhibits high conversion capacity on ginsenoside Rb1 into ginsenoside Rd. After 10 days of optimal reaction conditions for the crude enzyme, ginsenoside Rb1 is fully converted to ginsenoside Rd Paenibacillus sp. MBT213
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
crude enzyme of strain MBT213 exhibits high conversion capacity on ginsenoside Rb1 into ginsenoside Rd, under optimal conditions for 14 days, 3.794 mg/ml of ginsenoside Rb1 is fully hydrolyzed by crude enzyme of strain MBT213 Paenibacillus sp. MBT213

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rb1 + H2O
-
Paenibacillus sp. MBT213 ginsenoside Rd + D-glucopyranose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Paenibacillus sp. MBT213

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Paenibacillus sp. MBT213

pH Range

pH Minimum pH Maximum Comment Organism
5 8 activity range Paenibacillus sp. MBT213

General Information

General Information Comment Organism
physiological function ginsenoside Rd is the main hydrolyzed product of ginsenoside Rb1, Rb2, and Rc, which are contained more than 80% in ginseng roots. Also ginsenoside Rd is the major precursor of ginsenoside Rg3, Rh2, F2 and compound K, which have high biological activities Paenibacillus sp. MBT213