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Literature summary for 3.2.1.192 extracted from
- Substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides and its application in the production of ginsenosides Rg3, Rg2, and Rh1 from ginseng root extract (2015), J. Biosci. Bioeng., 119, 497-504 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Gordonia terrae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Gordonia terrae | |
| 2.46 | - |
ginsenoside Rb1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae |  |
| 2.74 | - |
ginsenoside Rd | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
| 14.66 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 156000 | - |
recombinant His-tagged enzyme, gel filtration | Gordonia terrae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Rb1 + H2O | Gordonia terrae | - |
ginsenoside Rd + D-glucose | - |
? | |
| ginsenoside Rd + H2O | Gordonia terrae | - |
ginsenoside Rg3 + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gordonia terrae | - |
- |
- |
| Gordonia terrae DSM 43249 | - |
- |
- |
| Gordonia terrae NBRC 100016 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Gordonia terrae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
effectivity of ginsenoside production, overview | Gordonia terrae |
| 16.4 | - |
purified recombinant enzyme, substrate ginsenoside Rd, pH 6.5, 30°C | Gordonia terrae |
| 250 | - |
purified recombinant enzyme, substrate ginsenoside Rb1, pH 6.5, 30°C | Gordonia terrae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Gordonia terrae | 2-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Gordonia terrae | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| ginsenoside Rb1 + H2O | - |
Gordonia terrae | ginsenoside Rd + D-glucose | - |
? | |
| ginsenoside Rd + H2O | - |
Gordonia terrae | ginsenoside Rg3 + D-glucose | - |
? | |
| additional information | substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside | Gordonia terrae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | see also EC 3.2.1.194 and EC 3.2.1.191 | Gordonia terrae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Gordonia terrae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | activity range, inactive above 50°C, recombinant enzyme | Gordonia terrae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.06 | - |
ginsenoside Rd | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
| 82.87 | - |
ginsenoside Rb1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
| 6292 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | Gordonia terrae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | about 60% of maximal activity pH 5.5 and pH 7.5 | Gordonia terrae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.21 | - |
ginsenoside Rd | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
| 33.69 | - |
ginsenoside Rb1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
| 1057.7 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
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