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Literature summary for 3.2.1.192 extracted from

  • Huq, M.; Siraj, F.; Kim, Y.; Yang, D.
    Enzymatic transformation of ginseng leaf saponin by recombinant beta-glucosidase (bgp1) and its efficacy in an adipocyte cell line (2016), Biotechnol. Appl. Biochem., 63, 532-538 .
No PubMed abstract available
Search for more literature for 3.2.1.192

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli strain BL21(DE3) Microbacterium esteraromaticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside F1 + H2O Microbacterium esteraromaticum reaction of EC 3.2.1.194 protopanaxatriol + D-glucose
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 ?
ginsenoside Rb1 + H2O Microbacterium esteraromaticum
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 ginsenoside Rg3 + 2 D-glucose
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 ?
ginsenoside Rd + H2O Microbacterium esteraromaticum
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 ginsenoside Rg3 + D-glucose
-
 ?
ginsenoside Re + H2O Microbacterium esteraromaticum reaction of EC 3.2.1.194 ginsenoside Rg2 + L-rhamnose
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 ?
ginsenoside Rg1 + H2O Microbacterium esteraromaticum reaction of EC 3.2.1.194 ginsenoside Rh1 + D-glucose
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 ?

Organism

Organism UniProt Comment Textmining
Microbacterium esteraromaticum A0A142G555
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside F1 + H2O reaction of EC 3.2.1.194 Microbacterium esteraromaticum protopanaxatriol + D-glucose
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 ?
ginsenoside Rb1 + H2O
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 Microbacterium esteraromaticum ginsenoside Rg3 + 2 D-glucose
-
 ?
ginsenoside Rd + H2O
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 Microbacterium esteraromaticum ginsenoside Rg3 + D-glucose
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 ?
ginsenoside Re + H2O reaction of EC 3.2.1.194 Microbacterium esteraromaticum ginsenoside Rg2 + L-rhamnose
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 ?
ginsenoside Rg1 + H2O reaction of EC 3.2.1.194 Microbacterium esteraromaticum ginsenoside Rh1 + D-glucose
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 ?
additional information the recombinant beta-glucosidase enzyme (bgp1) hydrolyzes all glucose moieties attached to the C-20 position of the ginsenosides Rg1, Re, Rb1, Rd, and F1. The products include pharmacologically active minor ginsenosides Rh1, Rg2, Rg3, F1, and protopanaxatriol. TLC and HPLC analysis of ginsenoside transformation by enzyme bgp1, overview Microbacterium esteraromaticum ?
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Synonyms

Synonyms Comment Organism
bgp1
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 Microbacterium esteraromaticum
bgp1C UniProt Microbacterium esteraromaticum
bgp1C UniProt, gene name Microbacterium esteraromaticum
More see also EC 3.2.1.194 Microbacterium esteraromaticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Microbacterium esteraromaticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Microbacterium esteraromaticum