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Literature summary for 3.2.1.191 extracted from

  • Shin, K.; Oh, D.
    Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides (2016), Crit. Rev. Biotechnol., 36, 1036-1049 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rb1 + H2O Sphingomonas sp.
-
ginsenoside G17 + D-glucose
-
?
ginsenoside Rb1 + H2O Sphingopyxis alaskensis
-
ginsenoside G17 + D-glucose
-
?
ginsenoside Rb1 + H2O Esteya vermicola via ginsenoside G17 ginsenoside G75 + 2 D-glucose
-
?
ginsenoside Rb1 + H2O Esteya vermicola CNU120806 via ginsenoside G17 ginsenoside G75 + 2 D-glucose
-
?
ginsenoside Rb2 + H2O Sphingomonas sp.
-
ginsenoside C-O + D-glucose
-
?
ginsenoside Rb2 + H2O Sphingopyxis alaskensis
-
ginsenoside C-O + D-glucose
-
?
ginsenoside Rc + H2O Sphingomonas sp.
-
ginsenoside C-Mc1 + D-glucose
-
?
ginsenoside Rc + H2O Sphingopyxis alaskensis
-
ginsenoside C-Mc1 + D-glucose
-
?
ginsenoside Rd + H2O Esteya vermicola via ginsenosside F2 ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O Sphingomonas sp.
-
ginsenoside F2 + D-glucose
-
?
ginsenoside Rd + H2O Sphingopyxis alaskensis
-
ginsenoside F2 + D-glucose
-
?
ginsenoside Rd + H2O Esteya vermicola CNU120806 via ginsenosside F2 ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rg3 + H2O Fusarium sp.
-
ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O Fusarium proliferatum
-
ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O Panax ginseng
-
ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O Sphingopyxis alaskensis
-
ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O Fusarium proliferatum ECU2042
-
ginsenoside Rh2 + D-glucose
-
?
additional information Aspergillus niger ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Fusarium sp. ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Sphingomonas sp. ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Fusarium proliferatum ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Panax ginseng ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Terrabacter ginsenosidimutans ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Sphingopyxis alaskensis ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Esteya vermicola ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Esteya vermicola CNU120806 ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?
additional information Fusarium proliferatum ECU2042 ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Esteya vermicola
-
-
-
Esteya vermicola CNU120806
-
-
-
Fusarium proliferatum
-
-
-
Fusarium proliferatum ECU2042
-
-
-
Fusarium sp.
-
-
-
Panax ginseng
-
-
-
Sphingomonas sp.
-
-
-
Sphingopyxis alaskensis
-
-
-
Terrabacter ginsenosidimutans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Esteya vermicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
pH 5.0, 60°C, substrate ginsenoside Rg3 Panax ginseng
76
-
purified enzyme, pH 7.0, 37°C Terrabacter ginsenosidimutans
290
-
purified native enzyme, pH 5.0, 50°C Esteya vermicola
309
-
pH 5.5, 40°C Sphingopyxis alaskensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rb1 + H2O
-
Sphingomonas sp. ginsenoside G17 + D-glucose
-
?
ginsenoside Rb1 + H2O
-
Sphingopyxis alaskensis ginsenoside G17 + D-glucose
-
?
ginsenoside Rb1 + H2O via ginsenoside G17 Terrabacter ginsenosidimutans ginsenoside G75 + 2 D-glucose
-
?
ginsenoside Rb1 + H2O via ginsenoside G17 Esteya vermicola ginsenoside G75 + 2 D-glucose
-
?
ginsenoside Rb1 + H2O via ginsenoside G17 Esteya vermicola CNU120806 ginsenoside G75 + 2 D-glucose
-
?
ginsenoside Rb2 + H2O
-
Sphingomonas sp. ginsenoside C-O + D-glucose
-
?
ginsenoside Rb2 + H2O
-
Sphingopyxis alaskensis ginsenoside C-O + D-glucose
-
?
ginsenoside Rb2 + H2O via ginsenoside C-O Terrabacter ginsenosidimutans ginsenoside C-Y + 2 D-glucose
-
?
ginsenoside Rb3 + H2O via ginsenoside C-Mx1 Terrabacter ginsenosidimutans ginsenoside C-Mx + 2 D-glucose
-
?
ginsenoside Rc + H2O
-
Sphingomonas sp. ginsenoside C-Mc1 + D-glucose
-
?
ginsenoside Rc + H2O
-
Sphingopyxis alaskensis ginsenoside C-Mc1 + D-glucose
-
?
ginsenoside Rc + H2O via ginsenoside C-Mc1 Terrabacter ginsenosidimutans ginsenoside C-Mc + 2 D-glucose
-
?
ginsenoside Rd + H2O via ginsenoside F2 Esteya vermicola ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O via ginsenosside F2 Terrabacter ginsenosidimutans ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O via ginsenosside F2 Esteya vermicola ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O via ginsenoside F2 Esteya vermicola CNU120806 ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O via ginsenosside F2 Esteya vermicola CNU120806 ginsenoside C-K + 2 D-glucose
-
?
ginsenoside Rd + H2O
-
Sphingomonas sp. ginsenoside F2 + D-glucose
-
?
ginsenoside Rd + H2O
-
Sphingopyxis alaskensis ginsenoside F2 + D-glucose
-
?
ginsenoside Rg3 + H2O
-
Fusarium sp. ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O
-
Fusarium proliferatum ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O
-
Panax ginseng ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O
-
Sphingopyxis alaskensis ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O
-
Fusarium proliferatum ECU2042 ginsenoside Rh2 + D-glucose
-
?
ginsenoside Rg3 + H2O via ginsenoside Rh2 Terrabacter ginsenosidimutans protopanaxadiol aglycone + 2 D-glucose
-
?
ginsenoside Rg3 + H2O via ginsenoside Rh2, hydrolysis of the inner glucose at C-3 Aspergillus niger protopanaxadiol aglycone + 2 D-glucose
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Aspergillus niger ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Fusarium sp. ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Sphingomonas sp. ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Fusarium proliferatum ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Panax ginseng ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Terrabacter ginsenosidimutans ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Sphingopyxis alaskensis ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Esteya vermicola ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Aspergillus niger ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Fusarium sp. ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Sphingomonas sp. ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Fusarium proliferatum ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Panax ginseng ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Terrabacter ginsenosidimutans ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Esteya vermicola ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides. beta-Glucosidase from Sphingopyxis alaskensis hydrolyzes only the outer glucose at C-3 in ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to product G17, C-O, C-Mc1, F2 and Rh2, respectively, without further hydrolysis Sphingopyxis alaskensis ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Esteya vermicola CNU120806 ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Esteya vermicola CNU120806 ?
-
?
additional information ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides Fusarium proliferatum ECU2042 ?
-
?
additional information ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides Fusarium proliferatum ECU2042 ?
-
?

Subunits

Subunits Comment Organism
? x * 51000 Sphingopyxis alaskensis
? x * 49000 Sphingomonas sp.
? x * 70000 Terrabacter ginsenosidimutans
? x * 59000 Panax ginseng

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
Sphingomonas sp.
beta-glucosidase
-
Panax ginseng
beta-glucosidase
-
Sphingopyxis alaskensis
beta-glucosidase
-
Esteya vermicola
ginsenosidase type III-ii
-
Aspergillus niger
ginsenosidase type III-ii
-
Fusarium sp.
ginsenosidase type III-ii
-
Sphingomonas sp.
ginsenosidase type III-ii
-
Fusarium proliferatum
ginsenosidase type III-ii
-
Panax ginseng
ginsenosidase type III-ii
-
Terrabacter ginsenosidimutans
ginsenosidase type III-ii
-
Sphingopyxis alaskensis
ginsenosidase type III-ii
-
Esteya vermicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Sphingomonas sp.
37
-
-
Terrabacter ginsenosidimutans
40
-
-
Sphingopyxis alaskensis
50
-
-
Esteya vermicola
60
-
-
Panax ginseng

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Sphingomonas sp.
5
-
-
Panax ginseng
5
-
-
Esteya vermicola
5.5
-
-
Sphingopyxis alaskensis
7
-
-
Terrabacter ginsenosidimutans

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 1, GH1 Sphingomonas sp.
evolution the enzyme belongs to the glycosyl hydrolase family 1, GH1 Sphingopyxis alaskensis
evolution the enzyme belongs to the glycosyl hydrolase family 3, GH3 Aspergillus niger
evolution the enzyme belongs to the glycosyl hydrolase family 3, GH3 Fusarium sp.
evolution the enzyme belongs to the glycosyl hydrolase family 3, GH3 Terrabacter ginsenosidimutans
evolution the enzyme belongs to the glycosyl hydrolase family 3, GH3 Esteya vermicola