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Literature summary for 3.2.1.189 extracted from

  • Fu, Y.; Yu, H.; Tang, S.H.; Hu, X.; Wang, Y.; Liu, B.; Yu, C.; Jin, F.
    New dioscin-glycosidase hydrolyzing multi-glycosides of dioscin from Absidia strain (2010), J. Microbiol. Biotechnol., 20, 1011-1017.
    View publication on PubMed
Search for more literature for 3.2.1.189

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, slight inhibition Absidia sp. (in: Fungi)
Cu2+ 10 mM, significant inhibition Absidia sp. (in: Fungi)
Hg2+ 10 mM, significant inhibition Absidia sp. (in: Fungi)

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity of the dioscin-glycosidase is not affected by Na+, K+, and Mg2+ at 50 mM Absidia sp. (in: Fungi)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Absidia sp. (in: Fungi)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O Absidia sp. (in: Fungi)
-
 D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O Absidia sp. (in: Fungi) d38
-
 D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?

Organism

Organism UniProt Comment Textmining
Absidia sp. (in: Fungi)
-
-
-
Absidia sp. (in: Fungi) d38
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Absidia sp. (in: Fungi)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.94
-
 40°C, pH 5.0 Absidia sp. (in: Fungi)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Absidia sp. (in: Fungi) D-glucopyranose + L-arabinose + L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Absidia sp. (in: Fungi) d38 D-glucopyranose + L-arabinose + L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Absidia sp. (in: Fungi) D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin is the the main dioscin in the rhizome of Dioscorea nipponica. The dioscin-glycosidase gradually hydrolyzes either the 3-O-alpha-L-(1->4)-Rha or the 3-O-alpha-L-(1->2)-Rha from dioscin into 3-O-alpha-L-Rha-(1->2)-beta-D-diosgenin or 3-O-alpha-L-Rha-(1->4)-beta-D-diosgenin, further rapidly hydrolyzes the other alpha-L-Rha into the main intermediate products of 3-O-beta-D-Glc-diosgenin, and subsequently hydrolyzes these intermediate product into aglycone as the final product Absidia sp. (in: Fungi) D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Absidia sp. (in: Fungi) d38 D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin is the the main dioscin in the rhizome of Dioscorea nipponica. The dioscin-glycosidase gradually hydrolyzes either the 3-O-alpha-L-(1->4)-Rha or the 3-O-alpha-L-(1->2)-Rha from dioscin into 3-O-alpha-L-Rha-(1->2)-beta-D-diosgenin or 3-O-alpha-L-Rha-(1->4)-beta-D-diosgenin, further rapidly hydrolyzes the other alpha-L-Rha into the main intermediate products of 3-O-beta-D-Glc-diosgenin, and subsequently hydrolyzes these intermediate product into aglycone as the final product Absidia sp. (in: Fungi) d38 D-glucopyranose + 2 L-rhamnopyranose + diosgenin
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Absidia sp. (in: Fungi)

Synonyms

Synonyms Comment Organism
dioscin glycosidase (aglycone-forming)
-
 Absidia sp. (in: Fungi)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Absidia sp. (in: Fungi)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 45% of maximal activity, 50°C: about 80% of maximal activity Absidia sp. (in: Fungi)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Absidia sp. (in: Fungi)

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 95% of maximal activity, pH 6.0: about 90% of maximal activity Absidia sp. (in: Fungi)