BRENDA - Enzyme Database
show all sequences of 3.2.1.188

Characterization of a membrane bound beta-glucosidase responsible for the activation of oat leaf saponins

Grünweller, S.; Kesselmeier, J.; Phytochemistry 24, 1941-1943 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition at 1 mM
Avena sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.183
-
avenacoside B
at pH 6.5 and 25°C
Avena sativa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
tonoplast
bound to the tonoplast membrane
Avena sativa
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
not influenced by 1 mM Cu2+
Avena sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
x * 68000, SDS-PAGE
Avena sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
avenacoside A + H2O
Avena sativa
-
26-desgluco-avenacoside A + D-glucose
-
-
?
avenacoside B + H2O
Avena sativa
-
26-desgluco-avenacoside B + D-glucose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Avena sativa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Avena sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrophenyl beta-D-glucopyranoside + H2O
-
726113
Avena sativa
4-nitrophenol + D-glucose
-
-
-
?
avenacoside A + H2O
-
726113
Avena sativa
26-desgluco-avenacoside A + D-glucose
-
-
-
?
avenacoside B + H2O
-
726113
Avena sativa
26-desgluco-avenacoside B + D-glucose
-
-
-
?
additional information
no activity with hederacoside C and cellobiose
726113
Avena sativa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 68000, SDS-PAGE
Avena sativa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Avena sativa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Avena sativa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.8
8
50% activity at pH 4.8 and 8.0, respectively
Avena sativa
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.5
-
at pH 6.5 and 25°C
Avena sativa
Hg2+
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.5
-
at pH 6.5 and 25°C
Avena sativa
Hg2+
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
complete inhibition at 1 mM
Avena sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.183
-
avenacoside B
at pH 6.5 and 25°C
Avena sativa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
tonoplast
bound to the tonoplast membrane
Avena sativa
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
not influenced by 1 mM Cu2+
Avena sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
x * 68000, SDS-PAGE
Avena sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
avenacoside A + H2O
Avena sativa
-
26-desgluco-avenacoside A + D-glucose
-
-
?
avenacoside B + H2O
Avena sativa
-
26-desgluco-avenacoside B + D-glucose
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Avena sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrophenyl beta-D-glucopyranoside + H2O
-
726113
Avena sativa
4-nitrophenol + D-glucose
-
-
-
?
avenacoside A + H2O
-
726113
Avena sativa
26-desgluco-avenacoside A + D-glucose
-
-
-
?
avenacoside B + H2O
-
726113
Avena sativa
26-desgluco-avenacoside B + D-glucose
-
-
-
?
additional information
no activity with hederacoside C and cellobiose
726113
Avena sativa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 68000, SDS-PAGE
Avena sativa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Avena sativa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Avena sativa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.8
8
50% activity at pH 4.8 and 8.0, respectively
Avena sativa
Other publictions for EC 3.2.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724918
Quidde
Evidence for three different s ...
Botrytis cinerea, Botrytis cinerea B05.10
Eur. J. Plant Pathol.
105
273-283
1999
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726124
Wubben
Detoxification of oat leaf sap ...
Parastagonospora avenae, Parastagonospora avenae WAC 1293
Phytopathology
86
986-992
1996
-
-
-
-
-
-
-
-
-
-
1
4
-
2
-
-
1
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
10
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
722249
Gus-Mayer
The amino acid sequence previo ...
Avena sativa
FEBS Lett.
347
51-54
1994
-
-
-
-
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
1
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723436
Gus-Mayer
Rüdiger, W.: Avenacosidase fr ...
Avena sativa
Plant Mol. Biol.
26
909-921
1994
-
-
-
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
4
1
-
9
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
4
1
-
9
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
726113
Grünweller
-
Characterization of a membrane ...
Avena sativa
Phytochemistry
24
1941-1943
1985
-
-
-
-
-
-
1
1
1
1
1
2
-
1
-
-
-
-
-
1
-
-
4
1
-
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
1
1
1
2
-
-
-
-
-
1
-
-
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-