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Literature summary for 3.2.1.188 extracted from

  • Gus-Mayer, S.; Brunner, H.; Schneider-Poetsch, H.A.; Lottspeich, F.; Eckerskorn, C.; Grimm, R.; Rüdiger, W.
    The amino acid sequence previously attributed to a protein kinase or a TCP1-related molecular chaperone and co-purified with phytochrome is a beta-glucosidase (1994), FEBS Lett., 347, 51-54.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
avenacoside B + H2O Avena sativa
-
26-desgluco-avenacoside B + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
cultivar Pirol
-

Purification (Commentary)

Purification (Comment) Organism
hydroxyapatite column chromatography Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.73
-
using 4-nitrophenyl-beta-galactoside as substrate, at pH 5.4 and 34°C for 15 min Avena sativa
2.72
-
using 4-nitrophenyl-beta-glucoside as substrate, at pH 5.4 and 34°C for 15 min Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactoside + H2O
-
Avena sativa 4-nitrophenol + D-galactopyranose
-
?
4-nitrophenyl beta-D-glucoside + H2O
-
Avena sativa 4-nitrophenol + D-glucopyranose
-
?
avenacoside B + H2O
-
Avena sativa 26-desgluco-avenacoside B + D-glucose
-
?
additional information no activity with 4-nitrophenyl-alpha-glucoside and 4-nitrophenyl-alpha-galactoside Avena sativa ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Avena sativa