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Literature summary for 3.2.1.183 extracted from

  • Vann, W.F.; Daines, D.A.; Murkin, A.S.; Tanner, M.E.; Chaffin, D.O.; Rubens, C.E.; Vionnet, J.; Silver, R.P.
    The NeuC protein of Escherichia coli K1 is a UDP N-acetylglucosamine 2-epimerase (2004), J. Bacteriol., 186, 706-712.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
neuC gene is cloned into an intein expression vector Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-alpha-D-acetylglucosamine + H2O enzyme catalyzes the formation of ManNAc from UDP-GlcNAc via a 2-acetamidoglucal intermediate Escherichia coli N-acetyl-alpha-D-mannosamine + UDP
-
 ?
UDP-N-alpha-D-acetylglucosamine + H2O enzyme catalyzes the formation of ManNAc from UDP-GlcNAc via a 2-acetamidoglucal intermediate Escherichia coli K1 N-acetyl-alpha-D-mannosamine + UDP
-
 ?

Synonyms

Synonyms Comment Organism
neuC
-
 Escherichia coli
UDP-N-acetylglucosamine 2-epimerase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction neuC is deleted from the chromosome of EV36, a K-12-K1 hybrid, by allelic exchange. Exogenously added sialic acid restores capsule expression to the deletion strain, confirming that NeuC is necessary for sialic acid synthesis. The NeuC homologue from serotype III Streptococcus agalactiae complements deletion mutant Escherichia coli