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Literature summary for 3.2.1.183 extracted from
- A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Purification and characterization of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (1997), J. Biol. Chem., 272, 24313-24318.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CMP-N-acetylneuraminic acid | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
monomer, SDS-PAGE, bifunctional enzymatic activity | Rattus norvegicus |
| 450000 | - |
gel filtration, dimer. Dimer does not show UDP-GlcNAc 2-epimerase activity. Incubation of the dimer with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer | Rattus norvegicus |
| 450000 | - |
gel filtration, hexamer, bifunctional enzyme complex | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using salmine sulfate precipitation and chromatography on phenyl-Sepharose, ATP-agarose, and Mono Q | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Rattus norvegicus | N-acetyl-alpha-D-mannosamine + UDP | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Rattus norvegicus Wistar | N-acetyl-alpha-D-mannosamine + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 75000, gel filtration | Rattus norvegicus |
| hexamer | 6 * 75000, gel filtration | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-GlcNAc 2-epimerase | bifunctional enzyme complex consisting of UDP-N-acetylglucosamine 2-epimerase and and N-acetylmannosamine kinase | Rattus norvegicus |
| UDP-N-acetylglucosamine 2-epimerase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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