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Literature summary for 3.2.1.181 extracted from

  • Kotake, T.; Hirata, N.; Degi, Y.; Ishiguro, M.; Kitazawa, K.; Takata, R.; Ichinose, H.; Kaneko, S.; Igarashi, K.; Samejima, M.; Tsumuraya, Y.
    Endo-beta-1,3-galactanase from winter mushroom Flammulina velutipes (2011), J. Biol. Chem., 286, 27848-27854.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Flammulina velutipes

Protein Variants

Protein Variants Comment Organism
E138D no activity Flammulina velutipes
E143D no activity Flammulina velutipes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beta-1,3-galactan the value for beta-1,3-galactan is 0.137 mg/ml Flammulina velutipes
0.57
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
1.25
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26690
-
calculated from amino acid sequence Flammulina velutipes
30000
-
SDS-PAGE Flammulina velutipes

Organism

Organism UniProt Comment Textmining
Flammulina velutipes F7J1C8 winter mushroom
-
Flammulina velutipes NBRC 7663 F7J1C8 winter mushroom
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Flammulina velutipes

Purification (Commentary)

Purification (Comment) Organism
-
Flammulina velutipes

Reaction

Reaction Comment Organism Reaction ID
The enzyme specifically hydrolyses beta-1,3-galactan and beta-1,3-galactooligosaccharides beta-1,4- and beta-1,6-galactooligosaccharides, agarose, or keratan sulfate do not serve as substrates Flammulina velutipes

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Flammulina velutipes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.8
-
pH 5.0, 37°C, liberation of D-galactose Flammulina velutipes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-galactan + H2O
-
Flammulina velutipes oligo-beta-(1->3)-D-galactose
-
?
beta-1,3-galactan + H2O
-
Flammulina velutipes NBRC 7663 oligo-beta-(1->3)-D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
?
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + D-galactopyranose after 30 min of reaction time the enzyme produces 42% D-glalactose, 30% dimeric, 20% trimeric and less than 10% tetrameric or pentameric beta-1,3-galactooligosaccharides ?
additional information The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. Flammulina velutipes ?
-
?
additional information The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. Flammulina velutipes NBRC 7663 ?
-
?

Synonyms

Synonyms Comment Organism
endo-beta-1,3-galactanase
-
Flammulina velutipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Flammulina velutipes
50
-
native enzyme Flammulina velutipes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.47
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
6.5
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6
-
Flammulina velutipes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
4.38
-
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes