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Literature summary for 3.2.1.18 extracted from

  • Prevato, M.; Ferlenghi, I.; Bonci, A.; Uematsu, Y.; Anselmi, G.; Giusti, F.; Bertholet, S.; Legay, F.; Telford, J.L.; Settembre, E.C.; Maione, D.; Cozzi, R.
    Expression and characterization of recombinant, tetrameric and enzymatically active influenza neuraminidase for the setup of an enzyme-linked lectin-based assay (2015), PLoS ONE, 10, e0135474 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Expi293F cells influenza A virus H5N1
expressed in Expi293F cells influenza A virus H1N1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02982
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H1N1
0.04493
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H5N1

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20 mM used in assay conditions. Calcium binding induces a large increase in the stability of the enzyme influenza A virus H5N1
Ca2+ 20 mM used in assay conditions. Calcium binding induces a large increase in the stability of the enzyme influenza A virus H1N1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 gel filtration influenza A virus H5N1
240000
-
 gel filtration influenza A virus H1N1

Organism

Organism UniProt Comment Textmining
influenza A virus H1N1 C7FH46 swine A/California/07/2009
-
influenza A virus H5N1 A5Z227 avian A/turkey/Turkey/01/2005
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 influenza A virus H5N1
glycoprotein
-
 influenza A virus H1N1

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity column chromatography, and Superdex gel filtration influenza A virus H5N1
HisTrap nickel affinity column chromatography, and Superdex gel filtration influenza A virus H1N1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 influenza A virus H5N1 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
 ?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 influenza A virus H1N1 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
 ?
fetuin + H2O
-
 influenza A virus H5N1 ?
-
 ?
fetuin + H2O
-
 influenza A virus H1N1 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 70000, SDS-PAGE influenza A virus H5N1
homotetramer 4 * 70000, SDS-PAGE influenza A virus H1N1
homotetramer 4 * 52000, calculated from amino acid sequence influenza A virus H5N1
homotetramer 4 * 52000, calculated from amino acid sequence influenza A virus H1N1

Synonyms

Synonyms Comment Organism
neuraminidase
-
 influenza A virus H5N1
neuraminidase
-
 influenza A virus H1N1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.58
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H1N1
75.45
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H5N1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1025
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H1N1
1679
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid at pH 5.5 and 37°C influenza A virus H5N1