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Literature summary for 3.2.1.18 extracted from

  • Huang, K.; Wang, M.M.; Kulinich, A.; Yao, H.L.; Ma, H.Y.; Martinez, J.E.; Duan, X.C.; Chen, H.; Cai, Z.P.; Flitsch, S.L.; Liu, L.; Voglmeir, J.
    Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila (2015), Carbohydr. Res., 415, 60-65 .
    View publication on PubMed
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Akkermansia muciniphila

Inhibitors

Inhibitors Comment Organism Structure
Co2+ isoforms Am0705 and Am2085 show complete inhibition at 2 mM Co2+, whereas isoforms Am0707 and Am1757 can maintain more than 60% of their enzymatic activities Akkermansia muciniphila
Cu2+ complete inhibition at 2 mM Akkermansia muciniphila
EDTA complete inhibition of isoforms Am0705, Am1757 and Am2085 at 2 mM. Isoform Am0707 retains 57.9% of its enzymatic activity after the addition of 2 mM EDTA Akkermansia muciniphila
Fe2+ 2 mM Fe2+ fully inactivates isoform Am2085 Akkermansia muciniphila
Fe3+ 2 mM Fe3+ decreases the activity of isoforms Am1757 and Am0707 to 54.5% and 68.2%, respectively Akkermansia muciniphila
Mg2+ 1 m Mg2+ reduces activities of isoforms Am0707 and Am2085 to 58.2% and 58.6%, respectively Akkermansia muciniphila
additional information isoform Am0707is not inhibited by EDTA Akkermansia muciniphila
Ni2+ 2 mM Ni2+ significantly decreases the activity of isoforms Am0705, Am0707 and Am1757 (44.3%, 28.5% and 5.3%, respectively) and fully inhibits isoform Am2085 Akkermansia muciniphila
Zn2+ complete inhibition at 2 mM Akkermansia muciniphila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am0707, at pH 6.5 and 37°C Akkermansia muciniphila
0.101
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am2085, at pH 6.5 and 37°C Akkermansia muciniphila
0.106
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am0705, at pH 6.5 and 37°C Akkermansia muciniphila
0.121
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am1757, at pH 6.5 and 37°C Akkermansia muciniphila

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 2 mM Fe2+ increases the activity of isoform Am0705 by 78% Akkermansia muciniphila
Fe3+ 2 mM Fe3+ increases the activity of isoform Am2085 to 145.1% Akkermansia muciniphila

Organism

Organism UniProt Comment Textmining
Akkermansia muciniphila
-
-
-
Akkermansia muciniphila DSM 22959
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Akkermansia muciniphila

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid + H2O
-
 Akkermansia muciniphila ?
-
 ?
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid + H2O
-
 Akkermansia muciniphila DSM 22959 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44400, isoform Am0707, calculated from amino acid sequence Akkermansia muciniphila
? x * 48100, isoform Am0705, calculated from amino acid sequence Akkermansia muciniphila
? x * 50000, isoforms Am0705 and Am0707, SDS-PAGE Akkermansia muciniphila
? x * 65600, isoform Am1757, calculated from amino acid sequence Akkermansia muciniphila
? x * 70000, isoforms Am1757 and Am2085, SDS-PAGE Akkermansia muciniphila
? x * 74300, isoform Am2085, calculated from amino acid sequence Akkermansia muciniphila

Synonyms

Synonyms Comment Organism
Am0705 isoform Akkermansia muciniphila
Am0707 isoform Akkermansia muciniphila
Am1757 isoform Akkermansia muciniphila
Am2085 isoform Akkermansia muciniphila
neuraminidase
-
 Akkermansia muciniphila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 the enzyme shows highest activities at temperatures between 37°C and 42°C, and decreases rapidly above 45°C Akkermansia muciniphila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am0705, at pH 6.5 and 37°C Akkermansia muciniphila
13.3
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am2085, at pH 6.5 and 37°C Akkermansia muciniphila
35
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am0707, at pH 6.5 and 37°C Akkermansia muciniphila
246
-
 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am1757, at pH 6.5 and 37°C Akkermansia muciniphila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 isoform Am0707 Akkermansia muciniphila
7
-
 isoform Am2085 Akkermansia muciniphila
7.5
-
 isoform Am1757 Akkermansia muciniphila
8
-
 isoform Am0705 Akkermansia muciniphila

pH Range

pH Minimum pH Maximum Comment Organism
4 9 isoform Am0707 shows considerable enzymatic activity between pH 4.0 and 9.0 Akkermansia muciniphila