Cloned (Comment) | Organism |
---|---|
expressed as GST-fusion protein in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D46A | inactive | Homo sapiens |
E218A | inactive | Homo sapiens |
E218Q | inactive | Homo sapiens |
K45A | the mutant shows about 3fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type | Homo sapiens |
Q112A | the mutant shows nearly 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type | Homo sapiens |
Q270A | the mutant shows about 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type | Homo sapiens |
Q270E | the mutant shows about wild type activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha(2-3)sialyl-lactose + H2O | Homo sapiens | - |
? | - |
? | |
monosialoganglioside GM3 + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y3R4 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose 4B column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Homo sapiens | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
alpha(2-3)sialyl-lactose + H2O | - |
Homo sapiens | ? | - |
? | |
monosialoganglioside GM3 + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acylneuraminosyl glycohydrolase | - |
Homo sapiens |
NEU2 | - |
Homo sapiens |
sialidase | - |
Homo sapiens |