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Literature summary for 3.2.1.18 extracted from

  • Mozzi, A.; Mazzacuva, P.; Zampella, G.; Forcella, M.E.; Fusi, P.A.; Monti, E.
    Molecular insight into substrate recognition by human cytosolic sialidase NEU2 (2012), Proteins, 80, 1123-1132.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as GST-fusion protein in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D46A inactive Homo sapiens
E218A inactive Homo sapiens
E218Q inactive Homo sapiens
K45A the mutant shows about 3fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
Q112A the mutant shows nearly 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
Q270A the mutant shows about 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
Q270E the mutant shows about wild type activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha(2-3)sialyl-lactose + H2O Homo sapiens
-
?
-
?
monosialoganglioside GM3 + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y3R4
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
Homo sapiens 4-methylumbelliferone + N-acetylneuraminic acid
-
?
alpha(2-3)sialyl-lactose + H2O
-
Homo sapiens ?
-
?
monosialoganglioside GM3 + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
N-acylneuraminosyl glycohydrolase
-
Homo sapiens
NEU2
-
Homo sapiens
sialidase
-
Homo sapiens