Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Trypanosoma rangeli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72700 | - |
x * 72700, calculated from amino acid sequence | Trypanosoma rangeli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fetuin + H2O | Trypanosoma rangeli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma rangeli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography, and gel filtration | Trypanosoma rangeli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-sialyllactose + H2O | - |
Trypanosoma rangeli | sialic acid + lactose | - |
? | |
casein glycomacropeptide + H2O | - |
Trypanosoma rangeli | sialic acid + ? | - |
? | |
casein glycomacropeptide + lactose | - |
Trypanosoma rangeli | 3'-sialyllactose + ? | - |
? | |
fetuin + H2O | - |
Trypanosoma rangeli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72700, calculated from amino acid sequence | Trypanosoma rangeli |
Synonyms | Comment | Organism |
---|---|---|
sialidase | - |
Trypanosoma rangeli |
Tr6 | - |
Trypanosoma rangeli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | engineered free sialidase Tr6 is stable at 25°C for at least 24 h. At 45°C the enzyme is stable for at least 100 min. 2% of the initial trans-sialidase activity is retained after 5 min of incubation at 65°C | Trypanosoma rangeli |