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Literature summary for 3.2.1.18 extracted from

  • Wu, Y.; Bi, Y.; Vavricka, C.J.; Sun, X.; Zhang, Y.; Gao, F.; Zhao, M.; Xiao, H.; Qin, C.; He, J.; Liu, W.; Yan, J.; Qi, J.; Gao, G.F.
    Characterization of two distinct neuraminidases from avian-origin human-infecting H7N9 influenza viruses (2013), Cell Res., 23, 1347-1355.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in an insect/baculovirus expression system H7N9 subtype

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES monohydrate pH 6.0 and 14% (w/v) polyethylene glycol 4000 H7N9 subtype
sitting drop vapor diffusion method, using 5% (v/v) 2-methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5 and 10% (w/v) polyethylene glycol 10000 H7N9 subtype

Protein Variants

Protein Variants Comment Organism
R294K the substitution confers neuraminidase inhibitor oseltamivir resistance, The mutation results in reduced enzyme catalytic efficiency along with lower viral fitness H7N9 subtype

Inhibitors

Inhibitors Comment Organism Structure
oseltamivir
-
 H7N9 subtype

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fetuin + H2O H7N9 subtype
-
 ?
-
 ?
fetuin + H2O H7N9 subtype A/Shanghai/1/2013
-
 ?
-
 ?
fetuin + H2O H7N9 subtype A/Anhui/1/2013
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
H7N9 subtype R4JGE3 influenza A virus
-
H7N9 subtype R4RZI6 influenza A virus
-
H7N9 subtype A/Anhui/1/2013 R4JGE3 influenza A virus
-
H7N9 subtype A/Shanghai/1/2013 R4RZI6 influenza A virus
-

Purification (Commentary)

Purification (Comment) Organism
-
 H7N9 subtype

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 H7N9 subtype 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 H7N9 subtype A/Shanghai/1/2013 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 H7N9 subtype A/Anhui/1/2013 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
fetuin + H2O
-
 H7N9 subtype ?
-
 ?
fetuin + H2O
-
 H7N9 subtype A/Shanghai/1/2013 ?
-
 ?
fetuin + H2O
-
 H7N9 subtype A/Anhui/1/2013 ?
-
 ?

Synonyms

Synonyms Comment Organism
neuraminidase
-
 H7N9 subtype