Any feedback?
Literature summary for 3.2.1.18 extracted from
- The sialidases of Clostridium perfringens type D strain CN3718 differ in their properties and sensitivities to inhibitors (2014), Appl. Environ. Microbiol., 80, 1701-1709.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | isoform NanI activity is enhanced by trypsin pretreatment and then only for substrates with an alpha-2,3- or alpha-2,6-sialic acid linkage | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 90% residual activity at 10 mM | Clostridium perfringens |  |
| Fe3+ | 56% residual activity at 10 mM | Clostridium perfringens | |
| additional information | not inhibited by EDTA | Clostridium perfringens | |
| N-acetyl-2,3-dehydro-2-deoxyneuraminic acid | - |
Clostridium perfringens | |
| p-chloromercuribenzoate | 18% residual activity at 10 mM | Clostridium perfringens | |
| siastatin B | - |
Clostridium perfringens | |
| Zn2+ | 61% residual activity at 10 mM | Clostridium perfringens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 110% activity at 10 mM | Clostridium perfringens | |
| Mg2+ | 115% activity at 10 mM | Clostridium perfringens | |
| Mn2+ | 110% activity at 10 mM | Clostridium perfringens | |
| additional information | not influenced by Fe2+ and Ni2+ | Clostridium perfringens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, isoform NanH, SDS-PAGE | Clostridium perfringens |
| 77000 | - |
x * 77000, isoform NanI, SDS-PAGE | Clostridium perfringens |
| 129000 | - |
x * 129000, isoform NanJ, SDS-PAGE | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
type D | - |
| Clostridium perfringens CN3718 | - |
type D | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Clostridium perfringens | 5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid | - |
? | |
| 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Clostridium perfringens CN3718 | 5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid | - |
? | |
| additional information | siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages | Clostridium perfringens | ? | - |
? | |
| additional information | siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages | Clostridium perfringens CN3718 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 129000, isoform NanJ, SDS-PAGE | Clostridium perfringens |
| ? | x * 43000, isoform NanH, SDS-PAGE | Clostridium perfringens |
| ? | x * 77000, isoform NanI, SDS-PAGE | Clostridium perfringens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NanH | isoform | Clostridium perfringens |
| NanI | isoform | Clostridium perfringens |
| NanJ | isoform | Clostridium perfringens |
| sialidase | - |
Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
isoform NanJ | Clostridium perfringens |
| 43 | - |
isoform NanH | Clostridium perfringens |
| 48 | - |
isoform NanI sialidase activity steadily increases with temperature until 48°C | Clostridium perfringens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the NanI sialidase shows no loss of activity after a 10 min incubation at 50°C in Tris-HCl buffer, pH 7.2 | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
at pH 9.0, the activity of all three sialidase isoforms has decreased by about 50% | Clostridium perfringens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0189 | - |
at pH 7.2 and 37°C | Clostridium perfringens | N-acetyl-2,3-dehydro-2-deoxyneuraminic acid | |
| 0.0422 | - |
at pH 7.2 and 37°C | Clostridium perfringens | siastatin B | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
