Crystallization (Comment) | Organism |
---|---|
wild-type and mutant D59A, complexed with 2 mM 3-deoxy-N-acetylneuraminic acid to give a covalent intermediate, in 2 M ammonium sulfate, 100 mM HEPES, pH 8.0, 2%PEG 400, used for microseeding, in 10% PEG 4000, 100 mM Tris-HCl, pH 7.5, and 5% isopropanol, soaking in buffer with 5 mM 2,3-difluoro-sialic acid at 25°C, soaking in buffer containing 10 mM alpha-(2-3)-sialyllactose or 2'(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, freezing and X-ray diffraction structure determination and analysis at 1.6 A | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
D59A | catalytic acid/base residue mutation, crystal structure determination, altered catalytic mechanism and enzyme-substrate structure compared to the wild-type enzyme, respectively | Trypanosoma cruzi |
additional information | introduction of 7 surface mutations does not alter the enzymes' activities but facilitate crystallization | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of Michaelis enzyme-substrate complexes | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha(2-3)-sialyllactose + H2O | Trypanosoma cruzi | - |
sialic acid + lactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
trans-sialidase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
colominic acid + H2O = sialic acid + lactose | trans-sialidase preferring the transfer of sialic acids from sialoglycoconjugates to beta-galactosyl acceptor molecules, ping-pong, double diplacement catalytic mechanism, Tyr342 is important, Asp59 is the acid/base residue, enzyme-substrate complex structure, covalent sialyl-enzyme intermediate | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Trypanosoma cruzi | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
alpha(2-3)-sialyllactose + H2O | - |
Trypanosoma cruzi | sialic acid + lactose | - |
? | |
alpha(2-3)-sialyllactose + H2O | formation of a covalent sialyl-enzyme intermediate | Trypanosoma cruzi | sialic acid + lactose | - |
? | |
additional information | the enzyme also performs transglycosylation reactions | Trypanosoma cruzi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the sialidase superfamily | Trypanosoma cruzi |
TcTS | - |
Trypanosoma cruzi |
trans-sialidase | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Trypanosoma cruzi |