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Literature summary for 3.2.1.18 extracted from
- Characterization of sialidase from Entamoaeba hystolitica and possible pathogenic role in amebiasis (2003), Parasitol. Res., 89, 302-307.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| galactose | activates | Entamoeba histolytica |  |
| lactose | activates | Entamoeba histolytica | |
| additional information | not affected by glucose, sucrose, and mannose, the motility of the organism is increased by about 6fold in vivo in presence of fetuin, alpha(2-3)-sialyllactose, and 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-didehydroneuraminic acid | - |
Entamoeba histolytica | |
| 4-nitrophenyl oxamic acid | - |
Entamoeba histolytica | |
| Cu2+ | - |
Entamoeba histolytica | |
| Fe2+ | - |
Entamoeba histolytica | |
| additional information | the motility of the organism is decreased in vivo in presence of 2,3-didehydroneuraminic acid and sialic acid containing compounds | Entamoeba histolytica | |
| Zn2+ | - |
Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.059 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 5.5, 37°C | Entamoeba histolytica | |
| 0.144 | - |
alpha(2-3)-sialyllactose | pH 5.5, 37°C | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Entamoeba histolytica | |
| Mg2+ | activates | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 677fold, to homogeneity by chromatography on hydroxyapatite, fetuin-agarose, and Mono Q | Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.05 | - |
purified enzyme | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Entamoeba histolytica | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
| alpha(2-3)-sialyllactose + H2O | - |
Entamoeba histolytica | sialic acid + lactose | - |
? | |
| alpha(2-6)-sialyllactose + H2O | low activity | Entamoeba histolytica | sialic acid + lactose | - |
? | |
| fetuin + H2O | - |
Entamoeba histolytica | sialic acid + ? | - |
? | |
| additional information | colominic acid, and gangliosides GM1 and GD1 are poor substrates | Entamoeba histolytica | ? | - |
? | |
| mucin + H2O | - |
Entamoeba histolytica | sialic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sialidase | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Entamoeba histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
2,3-didehydroneuraminic acid | pH 5.5, 37°C | Entamoeba histolytica | |
| 0.185 | - |
4-nitrophenyl oxamic acid | pH 5.5, 37°C | Entamoeba histolytica | |
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