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Literature summary for 3.2.1.18 extracted from

  • Mizan, S.; Henk, A.; Stallings, A.; Maier, M.; Lee, M.D.
    Cloning and characterization of sialidases with 2-6' and 2-3' sialyl lactose specificity from Pasteurella multocida (2000), J. Bacteriol., 182, 6874-6883.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
gene nanB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source Pasteurella multocida
genes nanH, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source Pasteurella multocida

Protein Variants

Protein Variants Comment Organism
additional information insertional inactivation of nanH results in a mutant, which is not deficient in sialidase production, but exhibits reduced activity Pasteurella multocida
additional information insertional inactivation of nanH, encoding the second sialidase of the organism, results in a mutant, which is not deficient in sialidase production, but exhibits reduced activity Pasteurella multocida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 alpha(2-3')-sialyllactose recombinant enzyme, pH 6.8, 37°C Pasteurella multocida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Pasteurella multocida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pasteurella multocida most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection ?
-
 ?
additional information Pasteurella multocida most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pasteurella multocida Q9EZV6 gene nanH, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview
-
Pasteurella multocida Q9EZV7 gene nanB, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli, 950fold, by ammonium sulfate precipitation, and several chromatographic steps Pasteurella multocida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Pasteurella multocida
1.9
-
 purified recombinant enzyme Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha(2-3')-sialyllactose + H2O nanH encoded enzyme is highly specific for the (2-3')-sialyllactose Pasteurella multocida sialic acid + lactose
-
 ?
alpha(2-3')-sialyllactose + H2O the nanB encoded enzyme hydrolyzes both 2-3'- and 2-6'-sialyllactose Pasteurella multocida sialic acid + lactose
-
 ?
alpha(2-6')-sialyllactose + H2O the nanB encoded enzyme hydrolyzes both (2-3')- and (2-6')-sialyllactose, the latter substrate is preferred Pasteurella multocida sialic acid + lactose
-
 ?
alpha-acid glycoprotein + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
apotransferrin + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
bovine submaxillary gland mucin + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
colominic acid + H2O i.e. (2-8')-sialyllactose Pasteurella multocida sialic acid + lactose
-
 ?
fetuin + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
ganglioside GD1a + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
ganglioside GT1b + H2O
-
 Pasteurella multocida sialic acid + ?
-
 ?
additional information substrate specificity Pasteurella multocida ?
-
 ?
additional information most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection Pasteurella multocida ?
-
 ?
additional information most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection Pasteurella multocida ?
-
 ?
additional information substrate specificity, no or poor activity with ganglioside GM1, colominic acid, and alpha(2-6')-sialyllactose Pasteurella multocida ?
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 80000, SDS-PAGE Pasteurella multocida

Synonyms

Synonyms Comment Organism
sialidase
-
 Pasteurella multocida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pasteurella multocida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.8 depending on the buffer system Pasteurella multocida

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
 Pasteurella multocida
5.5 9
-
 Pasteurella multocida