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Literature summary for 3.2.1.18 extracted from

  • Iriyama, N.; Takeuchi, N.; Shiraishi, T.; Izumi, K.; Sawada, M.T.; Takahashi, N.; Furuhata, K.; Ogura, H.; Uda, Y.
    Enzymatic properties of sialidase from the ovary of the starfish, Asterina pectinifera (2000), Comp. Biochem. Physiol. B, 126, 561-569.
    View publication on PubMed
Search for more literature for 3.2.1.18

Inhibitors

Inhibitors Comment Organism Structure
2,3-dehydro-2-deoxysialic acid 70.2% inhibition at 0.1 mM Patiria pectinifera
4-aminophenyl thioglucoside derivatives of sialic acid
-
 Patiria pectinifera
chondroitin sulfate A
-
 Patiria pectinifera
D-glucosamine 6-sulfate slight inhibition at 10 mM Patiria pectinifera
D-glucose 6-sulfate slight inhibition at 10 mM Patiria pectinifera
Dextran sulfate
-
 Patiria pectinifera
heparin
-
 Patiria pectinifera
methyl thioglucoside derivatives of sialic acid
-
 Patiria pectinifera
additional information no inhibition by 5-amino sialic acid and sialic acid methyl ester Patiria pectinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 6 * 55000, SDS-PAGE Patiria pectinifera
360000
-
 gel filtration Patiria pectinifera

Organism

Organism UniProt Comment Textmining
Patiria pectinifera
-
 starfish
-

Purification (Commentary)

Purification (Comment) Organism
12287fold, by ammonium precipitation, ultrafiltration, reverse phase, gel filtration and ConA-affinity chromatography, and preparative PAGE Patiria pectinifera

Reaction

Reaction Comment Organism Reaction ID
colominic acid + H2O = sialic acid + lactose enzyme does not act on 4-O-acetylated sialic acids Patiria pectinifera
a

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
 Patiria pectinifera
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.2
-
 purified enzyme Patiria pectinifera

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, sodium acetate, pH 4.5, stable for 1 year Patiria pectinifera
4°C, purified enzyme, sodium acetate, pH 4.5, stable for 2 months Patiria pectinifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Patiria pectinifera 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
alpha(2-3)-sialyllactose + H2O high activity Patiria pectinifera sialic acid + lactose
-
 ?
alpha(2-6)-sialyllactose + H2O
-
 Patiria pectinifera sialic acid + lactose
-
 ?
colominic acid + H2O
-
 Patiria pectinifera sialic acid + lactose
-
 ?
fetuin + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
ganglioside GD1a + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
ganglioside GD1b + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
ganglioside GM3 + H2O best substrate Patiria pectinifera sialic acid + ?
-
 ?
additional information ganglioside GM1 and bovine serum albumin are poor substrates, no activity with 4-methylumbelliferyl-alpha-3-deoxy-D-glycero-D-galacto-2-nonulosonic acid Patiria pectinifera ?
-
 ?
transferrin + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 55000, SDS-PAGE Patiria pectinifera

Synonyms

Synonyms Comment Organism
sialidase
-
 Patiria pectinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4
-
 Patiria pectinifera