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Literature summary for 3.2.1.18 extracted from

  • Inoue, S.; Lin, S.L.; Inoue, Y.; Groves, D.R.; Thomson, R.J.; von Itzstein, M.; Pavlova, N.V.; Li, S.C.; Li, Y.T.
    A unique sialidase that cleaves the Neu5Gcalpha2->5-OglycolylNeu5Gc linkage: comparison of its specificity with that of three microbial sialidases toward four sialic acid dimers (2001), Biochem. Biophys. Res. Commun., 280, 104-109.
    View publication on PubMed
Search for more literature for 3.2.1.18

Inhibitors

Inhibitors Comment Organism Structure
Neu5Ac2en inhibits competitively the hydrolysis of KDNalpha-(2-8)-KDN Crassostrea virginica
sialyl-2en inhibits competitively the hydrolysis of Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc Crassostrea virginica

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-
Crassostrea virginica
-
 oyster
-
Paenarthrobacter ureafaciens
-
-
-
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Crassostrea virginica

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Crassostrea virginica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Crassostrea virginica 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
alpha-sialyllactose + H2O
-
 Clostridium perfringens sialic acid + lactose
-
 ?
alpha-sialyllactose + H2O
-
 Paenarthrobacter ureafaciens sialic acid + lactose
-
 ?
alpha-sialyllactose + H2O
-
 Vibrio cholerae serotype O1 sialic acid + lactose
-
 ?
additional information no activity with sialyl-alpha-(2-8)-sialic acid Clostridium perfringens ?
-
 ?
additional information no activity with sialyl-alpha-(2-8)-sialic acid Paenarthrobacter ureafaciens ?
-
 ?
additional information no activity with sialyl-alpha-(2-8)-sialic acid Vibrio cholerae serotype O1 ?
-
 ?
additional information the dimers Neu5Gcalpha-(2-8)-Neu5Ac and Neu5Gcalpha-(2-8)-Neu5Gc are poor substrates Crassostrea virginica ?
-
 ?
Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc + H2O
-
 Clostridium perfringens 2 Neu5Gcalpha
-
 ?
Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc + H2O
-
 Paenarthrobacter ureafaciens 2 Neu5Gcalpha
-
 ?
Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc + H2O
-
 Vibrio cholerae serotype O1 2 Neu5Gcalpha
-
 ?
Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc + H2O
-
 Crassostrea virginica 2 Neu5Gcalpha
-
 ?
Neu5Gcalpha-(2-8)-Neu5Ac + H2O preferred substrate Clostridium perfringens Neu5Gc + Neu5Ac
-
 ?
Neu5Gcalpha-(2-8)-Neu5Ac + H2O preferred substrate Paenarthrobacter ureafaciens Neu5Gc + Neu5Ac
-
 ?
Neu5Gcalpha-(2-8)-Neu5Ac + H2O preferred substrate Vibrio cholerae serotype O1 Neu5Gc + Neu5Ac
-
 ?
Neu5Gcalpha-(2-8)-Neu5Gc + H2O
-
 Vibrio cholerae serotype O1 Neu5Gc + Neu5Gc
-
 ?
Neu5Gcalpha-(2-8)-Neu5Gc + H2O low activity Clostridium perfringens Neu5Gc + Neu5Gc
-
 ?
Neu5Gcalpha-(2-8)-Neu5Gc + H2O low activity Paenarthrobacter ureafaciens Neu5Gc + Neu5Gc
-
 ?
polysialic acid + H2O preference for cleavage of polymers with (-5-O-glycolylNeu5Gc2-)-units, also cleaves polymers with (-8Neu5Acalpha2-)- and (-8Neu5Gcalpha2-)-units Crassostrea virginica sialic acid
-
 ?
sialyl-alpha-(2-8)-sialic acid + H2O
-
 Crassostrea virginica sialic acid
-
 ?

Synonyms

Synonyms Comment Organism
sialidase
-
 Clostridium perfringens
sialidase
-
 Paenarthrobacter ureafaciens
sialidase
-
 Vibrio cholerae serotype O1
sialidase
-
 Crassostrea virginica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium perfringens
37
-
 assay at Paenarthrobacter ureafaciens
37
-
 assay at Vibrio cholerae serotype O1
37
-
 assay at Crassostrea virginica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1
-
 assay at Crassostrea virginica
5.5
-
 assay at Clostridium perfringens
5.5
-
 assay at Paenarthrobacter ureafaciens
5.8
-
 assay at Vibrio cholerae serotype O1