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Literature summary for 3.2.1.18 extracted from

  • Heuermann, D.; Roggentin, P.; Kleineidam, R.G.; Schauer, R.
    Purification and characterization of a sialidase from Clostridium chauvoei NC08596 (1991), Glycoconj. J., 8, 95-101.
    View publication on PubMed
Search for more literature for 3.2.1.18

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
 Clostridium chauvoei

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Clostridium chauvoei
Hg2+
-
 Clostridium chauvoei
Zn2+
-
 Clostridium chauvoei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
 Clostridium chauvoei
2
-
 N-acetylneuraminic acid-alpha-2,6-lactose
-
 Clostridium chauvoei
2.2
-
 N-acetylneuraminic acid-alpha-2,3-lactose
-
 Clostridium chauvoei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Clostridium chauvoei
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates the activity of the enzyme Clostridium chauvoei
Mg2+ stimulates the activity of the enzyme Clostridium chauvoei
Mn2+ stimulates the activity of the enzyme Clostridium chauvoei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 2 * 150000, SDS-PAGE Clostridium chauvoei
300000
-
 gel filtration Clostridium chauvoei

Organism

Organism UniProt Comment Textmining
Clostridium chauvoei
-
 NC08596
-
Clostridium chauvoei NC08596
-
 NC08596
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium chauvoei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.4
-
-
 Clostridium chauvoei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Clostridium chauvoei 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
 ?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Clostridium chauvoei NC08596 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
 ?
alpha-sialyllactose + H2O
-
 Clostridium chauvoei sialic acid + lactose
-
 ?
alpha-sialyllactose + H2O
-
 Clostridium chauvoei NC08596 sialic acid + lactose
-
 ?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
-
 Clostridium chauvoei N-acetylneuraminic acid + lactose
-
 ?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
-
 Clostridium chauvoei NC08596 N-acetylneuraminic acid + lactose
-
 ?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
-
 Clostridium chauvoei N-acetylneuraminic acid + lactose
-
 ?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
-
 Clostridium chauvoei NC08596 N-acetylneuraminic acid + lactose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 150000, SDS-PAGE Clostridium chauvoei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Clostridium chauvoei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 50
-
 Clostridium chauvoei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Clostridium chauvoei

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5
-
 Clostridium chauvoei