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Literature summary for 3.2.1.18 extracted from
- Diversity in the properties of two sialidase isoenzymes produced by Clostridium perfringens spp. (1995), Biol. Chem. Hoppe-Seyler, 376, 569-575.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | DSM 756, large salidase and A99, large salidase | Clostridium perfringens |  |
| 0.18 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | A99, small salidase, cloned | Clostridium perfringens | |
| 0.19 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | A99, small salidase | Clostridium perfringens | |
| 0.36 | - |
poly-alpha-2,8-lactose | A99, large salidase | Clostridium perfringens | |
| 0.5 | - |
poly-alpha-2,8-lactose | DSM 756, large salidase | Clostridium perfringens | |
| 0.53 | - |
sialyl-alpha-2,3-lactose | A99, small salidase | Clostridium perfringens | |
| 0.56 | - |
sialyl-alpha-2,3-lactose | A99, large salidase | Clostridium perfringens | |
| 0.58 | - |
sialyl-alpha-2,3-lactose | DSM 756, large salidase | Clostridium perfringens | |
| 1.7 | - |
sialyl-alpha-2,6-lactose | A99, large salidase | Clostridium perfringens | |
| 1.9 | - |
sialyl-alpha-2,3-lactose | A99, small salidase, cloned | Clostridium perfringens | |
| 2.2 | - |
sialyl-alpha-2,6-lactose | DSM 756, large salidase | Clostridium perfringens | |
| 2.8 | - |
sialyl-alpha-2,6-lactose | A99, small salidase, cloned | Clostridium perfringens | |
| 3.5 | - |
sialyl-alpha-2,6-lactose | A99, small salidase | Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Clostridium perfringens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration, small sialidase | Clostridium perfringens |
| 63000 | - |
gel filtration, higher molecular mass sialidase | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.03 | - |
A99, small sialidase | Clostridium perfringens |
| 390 | - |
A99, large sialidase | Clostridium perfringens |
| 570 | - |
DSM 756, large sialidase | Clostridium perfringens |
| 740 | - |
A99, small sialidase, cloned | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly-alpha-2,8-lactose + H2O | - |
Clostridium perfringens | ? | - |
? | |
| sialyl-alpha-2,3-lactose + H2O | - |
Clostridium perfringens | ? | - |
? | |
| sialyl-alpha-2,6-lactose + H2O | - |
Clostridium perfringens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
small sialidase | Clostridium perfringens |
| 51 | 55 | higher molecular mass sialidase | Clostridium perfringens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 55 | small sialidase | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
higher molecular mass sialidase | Clostridium perfringens |
| 6.1 | - |
small sialidase | Clostridium perfringens |
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