Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 46% inhibition | Bacillus sp. (in: Bacteria) | |
Co2+ | 1 mM, 22% inhibition | Bacillus sp. (in: Bacteria) | |
Cu2+ | 1 mM, 69% inhibition | Bacillus sp. (in: Bacteria) | |
Fe2+ | 1 mM, 94% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 1 mM, 77% inhibition | Bacillus sp. (in: Bacteria) | |
Zn2+ | 1 mM, 21% ihibition | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | Bacillus sp. (in: Bacteria) | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | Bacillus sp. (in: Bacteria) GL1 | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) GL1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:gellan-grown cell | - |
Bacillus sp. (in: Bacteria) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O | 114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O | 114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O | 25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O | 25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GlcNAc + H2O | 116% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GlcNAc | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | - |
Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
beta-D-4-deoxy-DELTA4,5-GlcAp2S-(1->3)-beta-D-GalNAc + H2O | 23.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | ? + beta-D-GalNAc | - |
? | |
additional information | the enzyme is specific for unsaturated glucuronides and inert to saturated glucuronides. The enzyme fails to liberate nitrophenol from p-nitrophenyl-beta-D-glucuronide. The enzyme prefers unsaturated chondroitin disaccharide without sulfate to those with sulfate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | the enzyme is specific for unsaturated glucuronides and inert to saturated glucuronides. The enzyme fails to liberate nitrophenol from p-nitrophenyl-beta-D-glucuronide. The enzyme prefers unsaturated chondroitin disaccharide without sulfate to those with sulfate | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 90% of maximal activity, 55°C: about 35% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 10 min, about 40% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | sodium acetate buffer | Bacillus sp. (in: Bacteria) |
Organism | Comment | Expression |
---|---|---|
Bacillus sp. (in: Bacteria) | induced in the bacterial cells grown on either gellan or xanthan. Expression levels of unsaturated glucuronyl hydrolase in the bacterium grown on gellan and xanthan are 20.7 and 16.4 mU/mg protein, respectively, while the bacterium grown in the glucose, pectin, or LB medium fails to produce the enzyme | up |