Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.84 | - |
Avicel | at pH 5.7 and 55°C | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus DSM1313 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ Sepharose column chromatography and Superdex S200 gel filtration | Acetivibrio thermocellus |
Renatured (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | 100% activity | Acetivibrio thermocellus | ? | - |
? | |
avicel + H2O | 100% activity | Acetivibrio thermocellus DSM1313 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Acetivibrio thermocellus DSM1313 | ? | - |
? | |
cellopentaose + H2O | about 75% activity compared to Avicel | Acetivibrio thermocellus | ? | - |
? | |
cellopentaose + H2O | about 75% activity compared to Avicel | Acetivibrio thermocellus DSM1313 | ? | - |
? | |
cellotetraose + H2O | about 20% activity compared to Avicel | Acetivibrio thermocellus | ? | - |
? | |
cellotetraose + H2O | about 20% activity compared to Avicel | Acetivibrio thermocellus DSM1313 | ? | - |
? | |
crystalline cellulose + H2O | preferred substrate | Acetivibrio thermocellus | ? | - |
? | |
additional information | no activity with pectin, xylan, cellobiose and cellotriose | Acetivibrio thermocellus | ? | - |
? | |
additional information | no activity with pectin, xylan, cellobiose and cellotriose | Acetivibrio thermocellus DSM1313 | ? | - |
? | |
phosphoric acid-swollen cellulose + H2O | about 30% activity compared to Avicel | Acetivibrio thermocellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Acetivibrio thermocellus |
? | x * 74268, His12-tagged GH domain, calculated from amino acid sequence | Acetivibrio thermocellus |
? | x * 74272, catalytic domain, HPLC-Q-TOF mass spectrometry | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
Cel48S | - |
Acetivibrio thermocellus |
exoglucanase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 80 | more than 70% activity between 55 and 80°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | more than 85% activity between pH 4.5 and 6.5, about 75% activity at pH 7.0 | Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
physiological function | major exoglucanase of the cellulosome | Acetivibrio thermocellus |