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Literature summary for 3.2.1.176 extracted from

  • Tuohy, M.G.; Walsh, D.J.; Murray, P.G.; Claeyssens, M.; Cuffe, M.M.; Savage, A.V.; Coughlan, M.P.
    Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii (2002), Biochim. Biophys. Acta, 1596, 366-380 .
    View publication on PubMed
Search for more literature for 3.2.1.176

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive; competitive Rasamsonia emersonii
lactose competitive; competitive Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 4-nitrophenyl-beta-D-lactoside pH 5, 50°C Rasamsonia emersonii
10.7
-
 4-nitrophenyl-beta-D-lactoside pH 5, 50°C Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
-
-
Rasamsonia emersonii Q8TFL9 isoform Cbh Ia
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 36-38% carbohydrate content Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 substrate 4-nitrophenyl-beta-lactoside, pH 5, 50°C Rasamsonia emersonii
2.5
-
 substrate 4-nitrophenyl-beta-lactoside, pH 5, 50°C Rasamsonia emersonii
24.8
-
 substrate avicel, pH 5, 50°C Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl-beta-D-cellotrioside + H2O
-
 Rasamsonia emersonii 2-chloro-4-nitrophenol + ?
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl-beta-D-lactoside
-
 Rasamsonia emersonii 4-nitrophenol + lactose
-
 ?
avicel + H2O
-
 Rasamsonia emersonii cellobiose + ?
-
 ?
cellulose + H2O
-
 Rasamsonia emersonii cellobiose + ?
-
 ?
additional information no hydrolysis of a soluble cellulose derivative and barley (1-3),(1-4)-beta-D-glucan Rasamsonia emersonii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56330, SDS-PAGE Rasamsonia emersonii
? x * 66100, SDS-PAGE Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
CBH Ib
-
 Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
66
-
-
 Rasamsonia emersonii
78
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life 34 min Rasamsonia emersonii
80
-
 half-life 68 min Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.4
-
 4-nitrophenyl-beta-D-lactoside pH 5.0, 50°C Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6
-
-
 Rasamsonia emersonii
4.1
-
-
 Rasamsonia emersonii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.18
-
 cellobiose pH 5.0, 50°C Rasamsonia emersonii
0.34
-
 lactose pH 5.0, 50°C Rasamsonia emersonii
2.5
-
 cellobiose pH 5.0, 50°C Rasamsonia emersonii
18.2
-
 lactose pH 5.0, 50°C Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii
-
-
 4.2