Literature summary for 3.2.1.176 extracted from

Dotsenko, A.; Gusakov, A.; Volkov, P.; Rozhkova, A.; Sinitsyn, A.
N-linked glycosylation of recombinant cellobiohydrolase I (Cel7A) from Penicillium verruculosum and its effect on the enzyme activity (2016), Biotechnol. Bioeng., 113, 283-291 .

Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Penicillium canescens strain PCA10	Talaromyces verruculosus

Protein Variants

Protein Variants	Comment	Organism
N194A	the substrate digestion rates of the mutant are notably lower relative to the wild type enzyme	Talaromyces verruculosus
N388A	the substrate digestion rates of the mutant are notably lower relative to the wild type enzyme	Talaromyces verruculosus
N45A	the mutation leads to a significant increase in the rate of Avicel and milled aspen wood hydrolysis compared to the wild type enzyme	Talaromyces verruculosus

Inhibitors

Inhibitors	Comment	Organism	Structure
cellobiose	-	Talaromyces verruculosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellulose + H2O	Talaromyces verruculosus	-	cellobiose + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Talaromyces verruculosus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme has four potential N-glycosylation sites at its catalytic module: Asn45, Asn194, Asn388, and Asn430	Talaromyces verruculosus

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Source 15Q column chromatography, and Source 15 isopropyl column chromatography	Talaromyces verruculosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.028	-	wild type enzyme, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.036	-	mutant enzyme N194A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.038	-	mutant enzyme N45A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.039	-	mutant enzyme N388A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.041	-	mutant enzyme N388A, with beta-glucan as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.044	-	mutant enzyme N194A, with beta-glucan as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.047	-	wild type enzyme, with beta-glucan as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.117	-	mutant enzyme N45A, with beta-glucan as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.17	-	mutant enzyme N194A, with Avicel as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.19	-	mutant enzyme N388A, with Avicel as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.2	-	wild type enzyme, with Avicel as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus
0.22	-	mutant enzyme N45A, with Avicel as substrate, at pH 4.0 and 40°C	Talaromyces verruculosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-lactoside + H2O	-	Talaromyces verruculosus	4-nitrophenol + beta-D-lactose	-	?
avicel + H2O	highest activity	Talaromyces verruculosus	?	-	?
beta-glucan + H2O	-	Talaromyces verruculosus	?	-	?
cellulose + H2O	-	Talaromyces verruculosus	cellobiose + ?	-	?
milled aspen wood + H2O	-	Talaromyces verruculosus	?	-	?
additional information	no activity with carboxymethyl cellulose, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl beta-D-galactopyranoside	Talaromyces verruculosus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 70000, SDS-PAGE	Talaromyces verruculosus

Synonyms

Synonyms	Comment	Organism
Cel7A	-	Talaromyces verruculosus
cellobiohydrolase I	-	Talaromyces verruculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	65	the enzyme is stable at 50°C at least for 3 h. At 60 and 65°C, the enzyme half-life times are in the range of 40-43 min and 11-13 min, respectively	Talaromyces verruculosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Talaromyces verruculosus

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Release 2023.1 (January 2023)