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Literature summary for 3.2.1.176 extracted from

  • Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
    Biochemical and structural characterizations of two Dictyostelium cellobiohydrolases from the amoebozoa kingdom reveal a high level of conservation between distant phylogenetic trees of life (2016), Appl. Environ. Microbiol., 82, 3395-3409 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Trichoderma reesei Dictyostelium discoideum
expressed in Trichoderma reesei Dictyostelium purpureum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium malonate, pH 7.0 and 12% (w/v) PEG 3350 Dictyostelium purpureum
sitting drop vapor diffusion method, using 12.5% (w/v) polyethylene glycol 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium HEPES-MOPS pH 7.5, 0.03 M MgCl2, and 0.03 M CaCl2 Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition Dictyostelium discoideum
cellobiose competitive inhibition Dictyostelium purpureum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium discoideum
3.4
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium purpureum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Dictyostelium discoideum
-
 cellobiose + ?
-
 ?
cellulose + H2O Dictyostelium purpureum
-
 cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q55FE6
-
-
Dictyostelium purpureum Q55FE6
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration Dictyostelium discoideum
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration Dictyostelium purpureum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Dictyostelium discoideum 4-nitrophenol + beta-D-lactose
-
 ?
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Dictyostelium purpureum 4-nitrophenol + beta-D-lactose
-
 ?
avicel + H2O
-
 Dictyostelium discoideum ?
-
 ?
avicel + H2O
-
 Dictyostelium purpureum ?
-
 ?
cellulose + H2O
-
 Dictyostelium discoideum cellobiose + ?
-
 ?
cellulose + H2O
-
 Dictyostelium purpureum cellobiose + ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium discoideum ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium purpureum ?
-
 ?
pretreated corn stover + H2O
-
 Dictyostelium discoideum ?
-
 ?
pretreated corn stover + H2O
-
 Dictyostelium purpureum ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel7A
-
 Dictyostelium discoideum
Cel7A
-
 Dictyostelium purpureum
cellobiohydrolase
-
 Dictyostelium discoideum
cellobiohydrolase
-
 Dictyostelium purpureum
GH7 CBH
-
 Dictyostelium discoideum
GH7 CBH
-
 Dictyostelium purpureum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Dictyostelium discoideum
55
-
-
 Dictyostelium purpureum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 melting temperature Dictyostelium discoideum
62
-
 melting temperature Dictyostelium purpureum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium discoideum
0.6
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium purpureum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Dictyostelium discoideum
5
-
-
 Dictyostelium purpureum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
 cellobiose at pH 5.0 and 40°C Dictyostelium purpureum
0.205
-
 cellobiose at pH 5.0 and 40°C Dictyostelium discoideum