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Literature summary for 3.2.1.176 extracted from

  • Xin, D.; Yang, M.; Chen, X.; Zhang, Y.; Ma, L.; Zhang, J.
    Improving the hydrolytic action of cellulases by Tween 80 offsetting the lost activity of cellobiohydrolase Cel7A (2017), ACS Sust. Chem. Eng., 5, 11339-11345 .
No PubMed abstract available
Search for more literature for 3.2.1.176

Activating Compound

Activating Compound Comment Organism Structure
Tween 80 specific activator, 20% increase of activity at 1 mM Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
additional information when the enzyme is supplemented with a mixture of inhibitors (0.5 mg/ml lignin, 0.5 mg/ml hemicelluloses, and 0.5 mM hemicellulose oligomers), 60% of the original cellobiohydrolase activity is lost Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 4-nitrophenyl D-cellobioside in the presence of 1 mM Tween 80, at pH 5.0 and 50°C Trichoderma reesei
0.81
-
 4-nitrophenyl D-cellobioside in the absence of Tween 80, at pH 5.0 and 50°C Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl D-cellobioside + H2O
-
 Trichoderma reesei 4-nitrophenol + cellobiose
-
 ?
aqueous ammonia-pretreated corn stover + H2O
-
 Trichoderma reesei ?
-
 ?
avicel + H2O
-
 Trichoderma reesei ?
-
 ?
cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
spruce + H2O
-
 Trichoderma reesei ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel7A
-
 Trichoderma reesei
cellobiohydrolase
-
 Trichoderma reesei