Literature summary for 3.2.1.176 extracted from

Haddad Momeni, M.; Ubhayasekera, W.; Sandgren, M.; Stahlberg, J.; Hansson, H.
Structural insights into the inhibition of cellobiohydrolase Cel7A by xylo-oligosaccharides (2015), FEBS J., 282, 2167-2177.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structures in complex with xylotriose, xylotetraose and xylopentaose reveal a predominant binding mode at the entrance of the substrate-binding tunnel of the enzyme, in which each xylose residue is shifted about 2.4 A towards the catalytic center compared with binding of cellooligosaccharides. Partial occupancy of two consecutive xylose residues at subsites -2 and -1 suggests an alternative binding mode for xylooligosaccharides in the vicinity of the catalytic center	Trichoderma reesei

Crystallization (Comment)

Organism

structures in complex with xylotriose, xylotetraose and xylopentaose reveal a predominant binding mode at the entrance of the substrate-binding tunnel of the enzyme, in which each xylose residue is shifted about 2.4 A towards the catalytic center compared with binding of cellooligosaccharides. Partial occupancy of two consecutive xylose residues at subsites -2 and -1 suggests an alternative binding mode for xylooligosaccharides in the vicinity of the catalytic center

Trichoderma reesei

Inhibitors

Inhibitors	Comment	Organism
birchwood xylan	mixed inhibition	Trichoderma reesei
additional information	xylose, Ki value above 1 mM	Trichoderma reesei
xylobiose	mixed inhibition	Trichoderma reesei
xylopentaose	mixed inhibition	Trichoderma reesei
xylotriose	mixed inhibition	Trichoderma reesei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	4-nitrophenyl lactoside	pH 5.0, 37°C	Trichoderma reesei

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	G0RVK1	-	-
Trichoderma reesei QM6a	G0RVK1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl lactoside + H2O	-	Trichoderma reesei	4-nitrophenol + lactose	-	?
4-nitrophenyl lactoside + H2O	-	Trichoderma reesei QM6a	4-nitrophenol + lactose	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
additional information	-	birchwood xylan	Kic 0.093 g/l, Kiu 0.18 g/l, pH 5.0, 37°C	Trichoderma reesei
0.0097	-	xylobiose	Kic, pH 5.0, 37°C	Trichoderma reesei
0.014	-	xylopentaose	Kic, pH 5.0, 37°C	Trichoderma reesei
0.026	-	xylopentaose	Kiu, pH 5.0, 37°C	Trichoderma reesei
0.029	-	xylotriose	Kic, pH 5.0, 37°C	Trichoderma reesei
0.056	-	xylotriose	Kiu, pH 5.0, 37°C	Trichoderma reesei
0.067	-	xylobiose	Kiu, pH 5.0, 37°C	Trichoderma reesei