Crystallization (Comment) | Organism |
---|---|
structures in complex with xylotriose, xylotetraose and xylopentaose reveal a predominant binding mode at the entrance of the substrate-binding tunnel of the enzyme, in which each xylose residue is shifted about 2.4 A towards the catalytic center compared with binding of cellooligosaccharides. Partial occupancy of two consecutive xylose residues at subsites -2 and -1 suggests an alternative binding mode for xylooligosaccharides in the vicinity of the catalytic center | Trichoderma reesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
birchwood xylan | mixed inhibition | Trichoderma reesei | |
additional information | xylose, Ki value above 1 mM | Trichoderma reesei | |
xylobiose | mixed inhibition | Trichoderma reesei | |
xylopentaose | mixed inhibition | Trichoderma reesei | |
xylotriose | mixed inhibition | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
4-nitrophenyl lactoside | pH 5.0, 37°C | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | G0RVK1 | - |
- |
Trichoderma reesei QM6a | G0RVK1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl lactoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + lactose | - |
? | |
4-nitrophenyl lactoside + H2O | - |
Trichoderma reesei QM6a | 4-nitrophenol + lactose | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
birchwood xylan | Kic 0.093 g/l, Kiu 0.18 g/l, pH 5.0, 37°C | Trichoderma reesei | |
0.0097 | - |
xylobiose | Kic, pH 5.0, 37°C | Trichoderma reesei | |
0.014 | - |
xylopentaose | Kic, pH 5.0, 37°C | Trichoderma reesei | |
0.026 | - |
xylopentaose | Kiu, pH 5.0, 37°C | Trichoderma reesei | |
0.029 | - |
xylotriose | Kic, pH 5.0, 37°C | Trichoderma reesei | |
0.056 | - |
xylotriose | Kiu, pH 5.0, 37°C | Trichoderma reesei | |
0.067 | - |
xylobiose | Kiu, pH 5.0, 37°C | Trichoderma reesei |