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Literature summary for 3.2.1.176 extracted from

  • Xin, D.; Ge, X.; Sun, Z.; Viikari, L.; Zhang, J.
    Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides (2015), Enzyme Microb. Technol., 68, 62-68.
    View publication on PubMed
Search for more literature for 3.2.1.176

Application

Application Comment Organism
degradation commercial cellulases contain mannan hydrolysing enzymes. Addition of 10 mg/ml mannan reduces the glucose yield of avicel (at 20 g/l) from 40.1 to 24.3%. The inhibitory effect is at least partly attributed to the inhibition of Cel7A(CBHI), but not on beta-glucosidase Thermoascus aurantiacus

Inhibitors

Inhibitors Comment Organism Structure
mannobiose competitive Thermoascus aurantiacus
Mannotetraose competitive Thermoascus aurantiacus
Mannotriose competitive Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C Thermoascus aurantiacus
0.76
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannotetraose Thermoascus aurantiacus
0.81
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannotriose Thermoascus aurantiacus
0.87
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannobiose Thermoascus aurantiacus
1.02
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 2 mM mannobiose Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus A7WNU2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + D-cellobiose
-
 ?

Synonyms

Synonyms Comment Organism
CBHI
-
 Thermoascus aurantiacus
Cel7A
-
 Thermoascus aurantiacus