Cloned (Comment) | Organism |
---|---|
expressed in yeast strain YDR483W BY4742 | Rasamsonia emersonii |
Protein Variants | Comment | Organism |
---|---|---|
A201P | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
A329G | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
A383Y | predicted from structure fold, calculation of the thermostability of the mutant, stabilizing mutation | Rasamsonia emersonii |
A383Y | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
D300K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
D354V | predicted from structure fold, calculation of the thermostability of the mutant, stabilizing mutation | Rasamsonia emersonii |
D354V | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
D52T | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
E325P | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
H208Y | predicted from structure fold, calculation of the thermostability of the mutant, stabilizing mutation | Rasamsonia emersonii |
H208Y | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
H358K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
H358R | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
H358V | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
L113M | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
additional information | prediction of point mutations to increase the thermostability of the enzyme, overview | Rasamsonia emersonii |
N126G | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
N439G | predicted from structure fold, calculation of the thermostability of the mutant, the mutation results in loss of expression | Rasamsonia emersonii |
N93K | predicted from structure fold, calculation of the thermostability of the mutant, increased thermotability compared to the wild-type enzyme | Rasamsonia emersonii |
N93K | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
P399G | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
Q345M | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
S130T | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
S13P | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
S13P/Y60L7S324P/A383Y7Y43 | predicted from structure fold, calculation of the thermostability of the mutant, the mutant is the most thermostable variant | Rasamsonia emersonii |
S222K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
S324P | predicted from structure fold, calculation of the thermostability of the mutant, stabilizing mutation | Rasamsonia emersonii |
S324P | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
S57D | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
S5T | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T164K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T257 V | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T257I | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T257K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T273K | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T273P | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T339P | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T339Q | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T392I | predicted from structure fold, calculation of the thermostability of the mutant, stabilizing mutation | Rasamsonia emersonii |
T392I | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
T395P | predicted from structure fold, calculation of the thermostability of the mutant, the mutation results in loss of expression | Rasamsonia emersonii |
T408D | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
T41V | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
V110L | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
V217I | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
V227L | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
V331M | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
V404A | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
Y430F | predicted from structure fold, calculation of the thermostability of the mutant, increased thermotability compared to the wild-type enzyme | Rasamsonia emersonii |
Y430F | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
Y60I | predicted from structure fold, calculation of the thermostability of the mutant | Rasamsonia emersonii |
Y60L | predicted from structure fold, calculation of the thermostability of the mutant, increased thermotability compared to the wild-type enzyme | Rasamsonia emersonii |
Y60L | the mutation increases stability and results in a 10°C increase in the optimal temperature for activity, to 65°C, and a 50% increase in total sugar production from crystalline cellulose compared to the wild type | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Rasamsonia emersonii | 4-methylumbelliferone + lactose | - |
? | |
crystalline cellulose + H2O | - |
Rasamsonia emersonii | cellobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBH I | - |
Rasamsonia emersonii |
Cel7A | - |
Rasamsonia emersonii |
cellobiohydrolase I | - |
Rasamsonia emersonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rasamsonia emersonii |