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Literature summary for 3.2.1.176 extracted from

  • Kern, M.; McGeehan, J.E.; Streeter, S.D.; Martin, R.N.; Besser, K.; Elias, L.; Eborall, W.; Malyon, G.P.; Payne, C.M.; Himmel, M.E.; Schnorr, K.; Beckham, G.T.; Cragg, S.M.; Bruce, N.C.; McQueen-Mason, S.J.
    Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance (2013), Proc. Natl. Acad. Sci. USA, 110, 10189-10194.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus oryzae and Aspergillus niger Limnoria quadripunctata
expression in Aspergillus oryzae and Aspergillus niger Limnoria quadripunctata

Crystallization (Commentary)

Crystallization (Comment) Organism
apo form at 1.6 A resolution, and three ligand bound complexes ranging from 1.9 A to 1.14 A resolution. Enzyme exhibits an extended substrate-binding motif at the tunnel entrance, which may aid in substrate acquisition and processivity. Enzyme stability and activity remain unchanged, or increase at high salt concentration Limnoria quadripunctata
vapor diffusion method, using 0.1 M CH3CO2Na pH 4.5, 15% (w/v) PEG 6000 and 0.5 M CaCl2 Limnoria quadripunctata

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl beta-D-cellopentaoside
-
 Limnoria quadripunctata
cellobiose
-
 Limnoria quadripunctata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Limnoria quadripunctata
45000
-
 gel filtration/multiangle laser light scattering Limnoria quadripunctata
46500
-
 calculated Limnoria quadripunctata

Organism

Organism UniProt Comment Textmining
Limnoria quadripunctata D4HRL0
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Trap column chromatography Limnoria quadripunctata

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
 Limnoria quadripunctata
-
hepatopancreas
-
 Limnoria quadripunctata
-
hepatopancreas secretion into gut for wood degradation Limnoria quadripunctata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O very low activity Limnoria quadripunctata 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellopentaoside + H2O best substrate Limnoria quadripunctata 4-nitrophenol + beta-D-cellopentaose
-
 ?
4-nitrophenyl beta-D-cellopentaoside + H2O
-
 Limnoria quadripunctata ?
-
 ?
4-nitrophenyl beta-D-cellotrioside + H2O
-
 Limnoria quadripunctata 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellotrioside + H2O
-
 Limnoria quadripunctata ?
-
 ?
avicel + H2O
-
 Limnoria quadripunctata ?
-
 ?
avicel + H2O
-
 Limnoria quadripunctata cellobiose + ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Limnoria quadripunctata ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Limnoria quadripunctata cellobiose + D-glucose + cellotriose
-
 ?

Subunits

Subunits Comment Organism
monomer 1* 45000, SDS-PAGE Limnoria quadripunctata

Synonyms

Synonyms Comment Organism
CBH
-
 Limnoria quadripunctata
Cel7B
-
 Limnoria quadripunctata
cellobiohydrolase
-
 Limnoria quadripunctata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Limnoria quadripunctata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 8 substrate 4-nitrophenyl beta-D-cellopentaoside Limnoria quadripunctata
4 8 with 4-nitrophenyl beta-D-cellopentaoside as substrate Limnoria quadripunctata
4 6.5 substrate 4-nitrophenyl beta-D-cellotrioside Limnoria quadripunctata
4 6.5 with 4-nitrophenyl beta-D-cellotrioside as substrate Limnoria quadripunctata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4
-
 cellobiose pH 4.5, 30°C Limnoria quadripunctata
1.4
-
 4-nitrophenyl beta-D-cellopentaoside at pH 4.5 and 30°C Limnoria quadripunctata