Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, apoenzyme using 20 mM MgCl2, 0.1 M HEPES, pH 7.5, 22% (w/v) polyacrylic acid 5100 sodium salt, or in complex with thio-beta-D-xylopentaose using 20mM MgCl2, 0.1 M HEPES, pH 7.7, 15-22% (w/v) polyethylene glycol 3350 | Heterobasidion irregulare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Heterobasidion irregulare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Heterobasidion irregulare | - |
cellobiose + ? | - |
? | |
cellulose + H2O | Heterobasidion irregulare TC-32-1 | - |
cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterobasidion irregulare | I1SB08 | - |
- |
Heterobasidion irregulare TC-32-1 | I1SB08 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange column chromatography and gel filtration | Heterobasidion irregulare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Heterobasidion irregulare | 4-methylumbelliferone + lactose | - |
? | |
4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Heterobasidion irregulare TC-32-1 | 4-methylumbelliferone + lactose | - |
? | |
cellulose + H2O | - |
Heterobasidion irregulare | cellobiose + ? | - |
? | |
cellulose + H2O | - |
Heterobasidion irregulare TC-32-1 | cellobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Heterobasidion irregulare |
Synonyms | Comment | Organism |
---|---|---|
Cel7A | - |
Heterobasidion irregulare |
cellobiohydrolase | - |
Heterobasidion irregulare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Heterobasidion irregulare |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | about 45% activity at 30°C, about 19% activity at 50°C | Heterobasidion irregulare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Heterobasidion irregulare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | about 65% activity at pH 3.0, 71% activity at pH 5.0, 23% activityat pH 6.0 | Heterobasidion irregulare |