Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.176 extracted from

  • Textor, L.C.; Colussi, F.; Silveira, R.L.; Serpa, V.; de Mello, B.L.; Muniz, J.R.; Squina, F.M.; Pereira, N.; Skaf, M.S.; Polikarpov, I.
    Joint X-ray crystallographic and molecular dynamics study of cellobiohydrolase I from Trichoderma harzianum: deciphering the structural features of cellobiohydrolase catalytic activity (2013), FEBS J., 280, 56-69.
    View publication on PubMed
Search for more literature for 3.2.1.176

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition; competitive inhibition Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
 4-nitrophenyl D-cellobioside pH 5.0, 50°C Trichoderma harzianum
3.4
-
 4-nitrophenyl beta-D-cellobioside 50 mM sodium acetate buffer, pH 5.0, at 50°C Trichoderma harzianum
3.7
-
 2-chloro-4-nitrophenyl-beta-lactoside pH 5.0, 50°C Trichoderma harzianum
3.7
-
 2-chloro-4-nitrophenyl beta-D-lactoside 50 mM sodium acetate buffer, pH 5.0, at 50°C Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma harzianum
-
 cellobiose + ?
-
 ?
cellulose + H2O Trichoderma harzianum IOC 3844
-
 cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum Q9P8P3
-
-
Trichoderma harzianum IOC 3844
-
-
-
Trichoderma harzianum IOC 3844 Q9P8P3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein predicted N-glycosylation sites at N126, N283 and N397 and the O-glycosylated linker, structure, overview Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl beta-D-lactoside + H2O
-
 Trichoderma harzianum 2-chloro-4-nitrophenol + lactose
-
 ?
2-chloro-4-nitrophenyl-beta-lactoside + H2O
-
 Trichoderma harzianum 2-chloro-4-nitrophenol + lactose
-
 ?
2-chloro-4-nitrophenyl-beta-lactoside + H2O
-
 Trichoderma harzianum IOC 3844 2-chloro-4-nitrophenol + lactose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma harzianum 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma harzianum IOC 3844 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl D-cellobioside + H2O
-
 Trichoderma harzianum 4-nitrophenol + D-cellobiose
-
 ?
4-nitrophenyl D-cellobioside + H2O
-
 Trichoderma harzianum IOC 3844 4-nitrophenol + D-cellobiose
-
 ?
avicel + H2O
-
 Trichoderma harzianum ?
-
 ?
avicel + H2O
-
 Trichoderma harzianum IOC 3844 ?
-
 ?
bacterial microcrystalline cellulose + H2O best substrate Trichoderma harzianum ?
-
 ?
bacterial microcrystalline cellulose + H2O best substrate Trichoderma harzianum IOC 3844 ?
-
 ?
carboxymethyl cellulose + H2O worst substrate Trichoderma harzianum ?
-
 ?
carboxymethyl cellulose + H2O worst substrate Trichoderma harzianum IOC 3844 ?
-
 ?
cellulose + H2O
-
 Trichoderma harzianum cellobiose + ?
-
 ?
cellulose + H2O
-
 Trichoderma harzianum IOC 3844 cellobiose + ?
-
 ?
additional information the enzyme is efficient in hydrolyzing crystalline cellulosic substrates, such as Avicel and Sigmacell 20, but is not effective in the hydrolysis of substituted substrates, such as caboxymethyl cellulose Trichoderma harzianum ?
-
 ?
additional information the enzyme is efficient in hydrolyzing crystalline cellulosic substrates, such as Avicel and Sigmacell 20, but is not effective in the hydrolysis of substituted substrates, such as caboxymethyl cellulose Trichoderma harzianum IOC 3844 ?
-
 ?
sigmacell 20 + H2O
-
 Trichoderma harzianum ?
-
 ?
sigmacell 20 + H2O
-
 Trichoderma harzianum IOC 3844 ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme molecular surface and structure analysis, overview Trichoderma harzianum

Synonyms

Synonyms Comment Organism
Cel7A
-
 Trichoderma harzianum
cellobiohydrolase I
-
 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Trichoderma harzianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
 4-nitrophenyl D-cellobioside pH 5.0, 50°C Trichoderma harzianum
0.39
-
 4-nitrophenyl beta-D-cellobioside 50 mM sodium acetate buffer, pH 5.0, at 50°C Trichoderma harzianum
0.74
-
 2-chloro-4-nitrophenyl-beta-lactoside pH 5.0, 50°C Trichoderma harzianum
0.74
-
 2-chloro-4-nitrophenyl beta-D-lactoside 50 mM sodium acetate buffer, pH 5.0, at 50°C Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Trichoderma harzianum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 cellobiose pH 5.0, 50°C Trichoderma harzianum
7.2
-
 cellobiose 50 mM sodium acetate buffer, pH 5.0, at 50°C Trichoderma harzianum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.2
-
 pH 5.0, 50°C Trichoderma harzianum cellobiose

General Information

General Information Comment Organism
metabolism Trichoderma harzianum secretes an effective and well-balanced enzymatic system that is able to completely hydrolyze cellulosic substrates into monomeric glucose Trichoderma harzianum
additional information cellobiohydrolase I has a shortened loop at the entrance of the cellulose-binding tunnel, the flexibility of Tyr260 is enhanced as a result of the short side-chains of adjacent Val216 and Ala384 residues and creates an additional gap at the side face of the catalytic tunnel. A larger flexibility in this region might facilitate the entry of the substrate into the tight binding tunnel of the enzyme. Structure analysis and molecular dynamics simulations, overview Trichoderma harzianum